TSP50 Polyclonal antibody 12574-1-AP proteintech
$149.00
In stock
SKU
12574-1-AP
| Catalog Number | 12574-1-AP |
| Synonyms | testes protease 50, Serine protease 50, PRSS50, EC:3.4.25.-, CT20 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | MCF7 cells, HEK-293 cells, human testis tissue, MCF-7 cells, mouse testis tissue |
| Tested Applications — Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2400 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Positive WB detected in | MCF7 cells, HEK-293 cells, human testis tissue, MCF-7 cells, mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue, human breast cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Western Blot (WB) | WB : 1:500-1:2400 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3247 Product name: Recombinant human TSP50 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 32-385 aa of BC033016 Sequence: LRSAGCWGAGEAPGALSTADPADQSVQCVPKATCPSSRPRLLWQTPTTQTLPSTTMETQFPVSEGKVDPYRSCGFSYEQDPTLRDPEAVARRWPWMVSVRANGTHICAGTIIASQWVLTVAHCLIWRDVIYSVRVGSPWIDQMTQTASDVPVLQVIMHSRYRAQRFWSWVGQANDIGLLKLKQELKYSNYVRPICLPGTDYVLKDHSRCTVTGWGLSKADGMWPQFRTIQEKEVIILNNKECDNFYHNFTKIPTLVQIIKSQMMCAEDTHREKFCYELTGEPLVCSMEGTWYLVGLVSWGAGCQKSEAPPIYLQVSSYQHWIWDCLNGQALALPAPSRTLLLALPLPLSLLAAL Predict reactive species |
| Full Name | testes-specific protease 50 |
| Calculated Molecular Weight | 385 aa, 43 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC033016 |
| Gene Symbol | TSP50 |
| Gene ID (NCBI) | 29122 |
| RRID | AB_10646475 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UI38 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TSP50 antibody 12574-1-AP | Download protocol |
| IHC protocol for TSP50 antibody 12574-1-AP | Download protocol |
| WB protocol for TSP50 antibody 12574-1-AP | Download protocol |
| human | IHC NPJ Biofilms Microbiomes TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome Authors - Xiaoli Li View Article |
| Citations | 5 |
| Dilutions | WB : 1:500-1:2400 IHC : 1:20-1:200 IF/ICC : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TSP50 (Testis-specific protease-like protein 50) is also named as PRSS50, CT20 and belongs to the peptidase S1 family. It can enhance the expression of NFKB target genes involved in cell proliferation, and promotes cell proliferation, at least partially, through activation of the NFKB signalling pathway(PMID:21385156). There are two protein bands of 37 kDa and 43 kDa to be detected in human testes that the the difference is most likely caused by posttranslational proteolytic cleavage(PMID:11782390). The full length protein has a signal peptide with 39 amino acids and two glycosylation sites. Protocols Product Specific Protocols IF protocol for TSP50 antibody 12574-1-AP Download protocol IHC protocol for TSP50 antibody 12574-1-AP Download protocol WB protocol for TSP50 antibody 12574-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Testes-specific protease 50 (TSP50) promotes invasion and metastasis by inducing EMT in gastric cancer.
Journal: BMC Cancer | Authors: Qing-Hua Cao KD Validated | Species: human | Application: WB,IHC - Lentivirus-mediated knockdown of TSP50 suppresses the growth of non-small cell lung cancer cells via G0/G1 phase arrest.
Journal: Oncol Rep | Authors: Wen-Liang Qiao - Overexpression of Testes-Specific Protease 50 (TSP50) Predicts Poor Prognosis in Patients with Gastric Cancer.
Journal: Gastroenterol Res Pract | Authors: Fang Liu | Species: human | Application: WB,IHC - Testes-specific protease 50 as an independent risk factor for poor prognosis in patients with non-small cell lung cancer.
Journal: Oncol Lett | Authors: Wen-Liang Qiao | Species: human | Application: IHC - TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome
Journal: NPJ Biofilms Microbiomes | Authors: Xiaoli Li | Species: human | Application: IHC
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