Gamma Adaptin Polyclonal antibody 13258-1-AP proteintech
$149.00
In stock
SKU
13258-1-AP
| Catalog Number | 13258-1-AP |
| Synonyms | AP1G1, Adaptor protein complex AP-1 subunit gamma-1, Adaptor-related protein complex 1 subunit gamma-1, ADTG, AP-1 complex subunit gamma-1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue |
| Tested Applications — Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | HEK-293 cells, mouse skeletal muscle tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3696 Product name: Recombinant human Gamma adaptin protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 476-825 aa of BC036283 Sequence: VQVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPTEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAPTSKPSSAGGELLDLLGDINLTGAPAAAPAPASVPQISQPPFLLDGLSSQPLFNDIAAGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSIVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ Predict reactive species |
| Full Name | adaptor-related protein complex 1, gamma 1 subunit |
| Calculated Molecular Weight | 825 aa, 92 kDa |
| Observed Molecular Weight | 91 kDa |
| GenBank Accession Number | BC036283 |
| Gene Symbol | Gamma Adaptin |
| Gene ID (NCBI) | 164 |
| RRID | AB_2058209 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43747 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Gamma Adaptin antibody 13258-1-AP | Download protocol |
| WB protocol for Gamma Adaptin antibody 13258-1-AP | Download protocol |
| human | WB bioRxiv Five inhibitory receptors display distinct vesicular distributions in T cells Authors - Jiahe Lu View Article |
| mouse | WB Front Cell Neurosci MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking. Authors - Xin-Tai Wang View Article |
| Citations | 7 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.
Journal: Nat Cell Biol | Authors: Boris Simonetti | Species: human | Application: WB - An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.
Journal: Proc Natl Acad Sci U S A | Authors: Yan Huang | Species: human | Application: IF - Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging.
Journal: Front Cell Dev Biol | Authors: Yan Huang | Species: human | Application: IF - Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells
Journal: Cells | Authors: Jiahe Lu | Species: human | Application: WB - MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking.
Journal: Front Cell Neurosci | Authors: Xin-Tai Wang | Species: mouse | Application: WB - Five inhibitory receptors display distinct vesicular distributions in T cells
Journal: bioRxiv | Authors: Jiahe Lu | Species: human | Application: WB - Five inhibitory receptors display distinct vesicular distributions in T cells
Journal: bioRxiv | Authors: Jiahe Lu | Species: human | Application: WB
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