UACA Polyclonal antibody 25654-1-AP proteintech
$149.00
In stock
SKU
25654-1-AP
| Catalog Number | 25654-1-AP |
| Synonyms | KIAA1561, NUCLING, Uveal autoantigen with coiled-coil domains and ankyrin repeats |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, canine |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A375 cells, HEK-293 cells, Neuro-2a cells, Y79 cells, HeLa cells |
| Tested Applications — Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A375 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Positive WB detected in | A375 cells, HEK-293 cells, Neuro-2a cells, Y79 cells, HeLa cells |
| Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, canine |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag22438 Product name: Recombinant human UACA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1075-1416 aa of BC113407 Sequence: LSQKYTEVKNVKEKLVEENAKQTSEILAVQNLLQKQHVPLEQVEALKKSLNGTIENLKEELKSMQRCYEKEQQTVTKLHQLLENQKNSSVPLAEHLQIKEAFEKEVGIIKASLREKEEESQNKMEEVSKLQSEVQNTKQALKKLETREVVDLSKYKATKSDLETQISSLNEKLANLNRKYEEVCEEVLHAKKKEISAKDEKELLHFSIEQEIKDQKERCDKSLTTITELQRRIQESAKQIEAKDNKITELLNDVERLKQALNGLSQLTYTSGNPTKRQSQLIDTLQHQVKSLEQQLADADRQHQEVIAIYRTHLLSAAQGHMDEDVQEALLQIIQMRQGLVC Predict reactive species |
| Full Name | uveal autoantigen with coiled-coil domains and ankyrin repeats |
| Calculated Molecular Weight | 1416 aa, 163 kDa |
| Observed Molecular Weight | 163 kDa |
| GenBank Accession Number | BC113407 |
| Gene Symbol | UACA |
| Gene ID (NCBI) | 55075 |
| RRID | AB_2880179 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZF9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for UACA antibody 25654-1-AP | Download protocol |
| IHC protocol for UACA antibody 25654-1-AP | Download protocol |
| WB protocol for UACA antibody 25654-1-AP | Download protocol |
| human | WB Cell Rep SUV39H1-dependent methyl-degron is a critical regulator of skeletal homeostasis. Authors - Qian Li View Article |
| mouse | IF J Zhejiang Univ Sci B Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification Authors - Tiantian Ge View Article |
| canine | WB Cell Rep Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells. Authors - Takahide Matsui View Article |
| Citations | 6 |
| Dilutions | WB : 1:2000-1:12000 IHC : 1:50-1:500 IF/ICC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells
Journal: Aging (Albany NY) | Authors: Hewei Zhang | Species: human | Application: WB - Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification
Journal: J Zhejiang Univ Sci B | Authors: Tiantian Ge | Species: mouse | Application: IF - Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling.
Journal: J Oncol | Authors: Yongqiang Wang | Species: human | Application: WB - Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells.
Journal: Cell Rep | Authors: Takahide Matsui | Species: canine | Application: WB - Knockdown of UACA inhibitsproliferation and invasion and promotes senescence of hepatocellular carcinoma cells
Journal: Int J Clin Exp Pathol | Authors: Jing-Yuan Sun KD Validated | Species: human | Application: WB,IF - SUV39H1-dependent methyl-degron is a critical regulator of skeletal homeostasis.
Journal: Cell Rep | Authors: Qian Li | Species: human | Application: WB
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