UBE2D1/2/3/4 Polyclonal antibody 15475-1-AP proteintech
$149.00
In stock
SKU
15475-1-AP
| Catalog Number | 15475-1-AP |
| Synonyms | HBUCE1, UBCH5D, UBE2D1, UBE2D1/2/3/4, UBE2D2, UBE2D3, UBE2D4, Ubiquitin carrier protein D4, Ubiquitin protein ligase D4 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, CoIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, human testis tissue, rat brain tissue |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Positive WB detected in | mouse brain tissue, human testis tissue, rat brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7779 Product name: Recombinant human UBE2D4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC004104 Sequence: MALKRIQKELTDLQRDPPAQCSAGPVGDDLFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPEIAHTYKADREKYNRLAREWTQKYAM Predict reactive species |
| Full Name | ubiquitin-conjugating enzyme E2D 4 (putative) |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC004104 |
| Gene Symbol | UBE2D4 |
| Gene ID (NCBI) | 51619 |
| RRID | AB_2210167 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2X8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for UBE2D1/2/3/4 antibody 15475-1-AP | Download protocol |
| WB protocol for UBE2D1/2/3/4 antibody 15475-1-AP | Download protocol |
| human,mouse | WB Int Dent J LncRNA AFAP1-AS1 Promotes Oral Squamous Cell Carcinoma Development by Ubiquitin-Mediated Proteolysis Authors - Bao-Jun Li View Article |
| rat | WB Environ Toxicol Blood RNA-sequencing analysis in acrylamide-induced neurotoxicity and depressive symptoms in rats Authors - Yng-Tay Chen View Article |
| human | IHC Transl Androl Urol Systematic analysis of UBE2D3 and its association with prognosis, tumor microenvironment, and drug sensitivity in renal clear cell carcinoma. Authors - Yichen Ye View Article |
| Citations | 6 |
| Dilutions | WB : 1:500-1:3000 IHC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology. Protocols Product Specific Protocols IHC protocol for UBE2D1/2/3/4 antibody 15475-1-AP Download protocol WB protocol for UBE2D1/2/3/4 antibody 15475-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The E3 ubiquitin ligase FBXL18 stabilizes BST2 to promote inflammation in RABV-infected astrocytes
Journal: Nat Commun | Authors: Jie Wang - LncRNA AFAP1-AS1 Promotes Oral Squamous Cell Carcinoma Development by Ubiquitin-Mediated Proteolysis
Journal: Int Dent J | Authors: Bao-Jun Li | Species: human,mouse | Application: WB - Blood RNA-sequencing analysis in acrylamide-induced neurotoxicity and depressive symptoms in rats
Journal: Environ Toxicol | Authors: Yng-Tay Chen | Species: rat | Application: WB - Silencing of UBE2D1 inhibited cell migration in gastric cancer, decreasing ubiquitination of SMAD4.
Journal: Infect Agent Cancer | Authors: Honghu Xie KD Validated | Species: human | Application: WB,CoIP - Systematic analysis of UBE2D3 and its association with prognosis, tumor microenvironment, and drug sensitivity in renal clear cell carcinoma.
Journal: Transl Androl Urol | Authors: Yichen Ye | Species: human | Application: IHC - Analysis and validation of abnormal signaling pathways and immune cell infiltration characteristics in digestive system cancers based on peroxisome-related genes.
Journal: Biol Direct | Authors: Jie Wen
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