UBE2R2 Polyclonal antibody 14077-1-AP proteintech
$149.00
In stock
SKU
14077-1-AP
| Catalog Number | 14077-1-AP |
| Synonyms | CDC34B, E2 ubiquitin-conjugating enzyme R2, EC:2.3.2.23, UBC3B, Ubiquitin carrier protein R2 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Jurkat cells, HeLa cells, mouse spleen tissue |
| Tested Applications — Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | Jurkat cells, HeLa cells, mouse spleen tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5202 Product name: Recombinant human UBE2R2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-238 aa of BC047584 Sequence: MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPYSPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDLYDDDIDDEDEEEEDADCYDDDDSGNEES Predict reactive species |
| Full Name | ubiquitin-conjugating enzyme E2R 2 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC047584 |
| Gene Symbol | UBE2R2 |
| Gene ID (NCBI) | 54926 |
| RRID | AB_2878010 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q712K3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for UBE2R2 antibody 14077-1-AP | Download protocol |
| IHC protocol for UBE2R2 antibody 14077-1-AP | Download protocol |
| WB protocol for UBE2R2 antibody 14077-1-AP | Download protocol |
| human | WB bioRxiv Shared and Distinct Mechanisms of UBA1 Inactivation Across Different Diseases Authors - Jason C Collins View Article |
| mouse | WB JCI Insight PMID: 31292296 Authors - Isabelle K Vila View Article |
| Citations | 5 |
| Dilutions | WB : 1:500-1:1000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- BiDAC-dependent degradation of plasma membrane proteins by the endolysosomal system
Journal: Nat Commun | Authors: Sammy Villa | Application: WB - Shared and distinct mechanisms of UBA1 inactivation across different diseases
Journal: EMBO J | Authors: Jason C Collins | Species: human | Application: WB - PMID: 31292296
Journal: JCI Insight | Authors: Isabelle K Vila | Species: mouse | Application: WB - p53 accelerates endothelial cell senescence in diabetic retinopathy by enhancing FoxO3a ubiquitylation and degradation via UBE2L6
Journal: Exp Gerontol | Authors: Ying Cheng | Species: human | Application: WB
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