UBE2T/HSPC150 Recombinant monoclonal antibody 86278-1-RR proteintech
$149.00
In stock
SKU
86278-1-RR
| Catalog Number | 86278-1-RR |
| Synonyms | HSPC150, UBE2T, Cell proliferation-inducing gene 50 protein, E2 ubiquitin-conjugating enzyme T, EC:2.3.2.23 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, SKOV-3 cells, Jurkat cells, K-562 cells, SW480 cells |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | HeLa cells, HepG2 cells, SKOV-3 cells, Jurkat cells, K-562 cells, SW480 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag0153 Product name: Recombinant human UBE2T protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 2-190 aa of BC004152 Sequence: QRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIE Predict reactive species |
| Full Name | ubiquitin-conjugating enzyme E2T (putative) |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC004152 |
| Gene Symbol | UBE2T |
| Gene ID (NCBI) | 29089 |
| RRID | AB_3744776 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NPD8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for UBE2T/HSPC150 antibody 86278-1-RR | Download protocol |
| WB protocol for UBE2T/HSPC150 antibody 86278-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250784D6 |
| Conjugation | Unconjugated |
uitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways. Protocols Product Specific Protocols IHC protocol for UBE2T/HSPC150 antibody 86278-1-RR Download protocol WB protocol for UBE2T/HSPC150 antibody 86278-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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