MMS2 Polyclonal antibody 10689-1-AP proteintech
$149.00
In stock
SKU
10689-1-AP
| Catalog Number | 10689-1-AP |
| Synonyms | UBE2V2, DDVit 1, DDVIT1, EDAF 1, EDAF-1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A375 cells, human brain tissue, rat liver tissue, HEK-293 cells, HL-60 cells, HeLa cells, Raji cells |
| Tested Applications — Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Positive WB detected in | A375 cells, human brain tissue, rat liver tissue, HEK-293 cells, HL-60 cells, HeLa cells, Raji cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1111 Product name: Recombinant human UBE2V2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC007051 Sequence: MAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN Predict reactive species |
| Full Name | ubiquitin-conjugating enzyme E2 variant 2 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC007051 |
| Gene Symbol | MMS2 |
| Gene ID (NCBI) | 7336 |
| RRID | AB_2211656 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15819 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for MMS2 antibody 10689-1-AP | Download protocol |
| IHC protocol for MMS2 antibody 10689-1-AP | Download protocol |
| WB protocol for MMS2 antibody 10689-1-AP | Download protocol |
| mouse,human | WB,IF Transl Stroke Res MicroRNA-193a-5p Rescues Ischemic Cerebral Injury by Restoring N2-Like Neutrophil Subsets Authors - Ziping Han View Article |
| human | WB,IF Biochim Biophys Acta Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection. Authors - Fan Chai View Article |
| Citations | 2 |
| Dilutions | WB : 1:1000-1:8000 IHC : 1:400-1:1600 IF/ICC : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
MMS2 encodes a 137-amino acid, 15.2-kDa protein with significant sequence homology to a conserved family of ubiquitin-conjugating (Ubc) proteins (PMID: 9576943). Mms2 forms a specific complex with the UBC13-encoded E2, and this complex assembles polyubiquitin chains linked through lysine 63 (PMID: 10089880). Mms2 is a ubiquitin E2-variant protein with a very well-documented function in the tolerance pathway that protects both human and yeast cells from the lethal and mutagenic effects of DNA damage (PMID: 36934633). A high expression level of human MMS2 is associated with poor survival prognosis in different cancer diseases (PMID: 36934633). Protocols Product Specific Protocols IF protocol for MMS2 antibody 10689-1-AP Download protocol IHC protocol for MMS2 antibody 10689-1-AP Download protocol WB protocol for MMS2 antibody 10689-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- MicroRNA-193a-5p Rescues Ischemic Cerebral Injury by Restoring N2-Like Neutrophil Subsets
Journal: Transl Stroke Res | Authors: Ziping Han | Species: mouse,human | Application: WB,IF - Subcellular quantitative proteomic analysis reveals host proteins involved in human cytomegalovirus infection.
Journal: Biochim Biophys Acta | Authors: Fan Chai | Species: human | Application: WB,IF
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