Multi-rAb® UCP1 Multi-Recombinant antibody RMX00036 proteintech
$449.00
In stock
SKU
RMX00036
| Catalog Number | RMX00036 |
| Synonyms | SLC25A7, Thermogenin, UCP, UCP 1 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brown adipose tissue |
| Tested Applications — Positive IP detected in | mouse brown adipose tissue |
| Tested Applications — Positive IHC detected in | mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | mouse brown adipose tissue |
| Positive IP detected in | mouse brown adipose tissue |
| Positive IHC detected in | mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Class | Multi-Recombinant |
| Type | Antibody |
| Immunogen | Mixture Predict reactive species |
| Full Name | uncoupling protein 1 (mitochondrial, proton carrier) |
| Calculated Molecular Weight | 307 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC098168 |
| Gene Symbol | UCP1 |
| Gene ID (NCBI) | 7350 |
| RRID | AB_3750090 |
| Conjugate | Unconjugated |
| Purification Method | N/A |
| UNIPROT ID | P25874 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for UCP1 antibody RMX00036 | Download protocol |
| IHC protocol for UCP1 antibody RMX00036 | Download protocol |
| IP protocol for UCP1 antibody RMX00036 | Download protocol |
| WB protocol for UCP1 antibody RMX00036 | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Multi-Recombinant |
| Clone Number | - |
| Conjugation | Unconjugated |
UCP-1 (Mitochondrial uncoupling protein 1), is a mitochondrial transporter protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. It has been identified a key molecule for metabolic thermogenesis to avoid an excess of fat accumulation. UCP-1 expression is usually restricted to brown adipose when induced by cold exposure and thyroid hormone. Protocols Product Specific Protocols IF protocol for UCP1 antibody RMX00036 Download protocol IHC protocol for UCP1 antibody RMX00036 Download protocol IP protocol for UCP1 antibody RMX00036 Download protocol WB protocol for UCP1 antibody RMX00036 Download protocol Standard Protocols Click here to view our Standard Protocols
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