UNC45A Polyclonal antibody 19564-1-AP proteintech
$149.00
In stock
SKU
19564-1-AP
| Catalog Number | 19564-1-AP |
| Synonyms | GC UNC45, GCUNC 45, GCUNC45, GCUNC-45, IRO039700 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | K-562 cells, mouse lung tissue, mouse ovary tissue, HeLa cells |
| Tested Applications — Positive IP detected in | K-562 cells |
| Tested Applications — Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Positive WB detected in | K-562 cells, mouse lung tissue, mouse ovary tissue, HeLa cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | unc-45 homolog A (C. elegans) |
| Calculated Molecular Weight | 103 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | NM_018671 |
| Gene Symbol | UNC45A |
| Gene ID (NCBI) | 55898 |
| RRID | AB_10644335 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for UNC45A antibody 19564-1-AP | Download protocol |
| IP protocol for UNC45A antibody 19564-1-AP | Download protocol |
| WB protocol for UNC45A antibody 19564-1-AP | Download protocol |
| mouse | IHC,IF Cells UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System. Authors - Valentino Clemente View Article |
| human | WB FASEB J A myosin chaperone, UNC-45A, is a novel regulator of intestinal epithelial barrier integrity and repair. Authors - Susana Lechuga KO Validated View Article |
| rat | WB,IF J Cell Sci UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II. Authors - Juri Habicht View Article |
| Kishor (Verified Customer) (10-17-2019) | This Ab works well for western blotting (1:1000) but I did not get satisfactory results for Immunofluorescence (1:200) in human liver cell lines. Applications: Western Blot, Immunofluorescence, Primary Antibody Dilution: 1:1000 and 1:200 Cell Tissue Type: H69, HUCCT-1 UNC45A Antibody Western Blot,Immunofluorescence, validation (1:1000 and 1:200 dilution) in H69, HUCCT-1 (Cat no:19564-1-AP) |
| Citations | 3 |
| Dilutions | WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
UNC45A, also named as SMAP1, acts as co-chaperone for HSP90. UNC45A prevents the stimulation of HSP90AB1 ATPase activity by AHSA1. It is a positive factor in promoting PGR function in the cell. It is necessary for proper folding of myosin and necessary for normal cell proliferation, normal myotube formation and myosin accumulation during muscle cell development. UNC45A plays a role in erythropoiesis in stroma cells in the spleen. The antibody recognizes the C-term of UNC45A. Protocols Product Specific Protocols IHC protocol for UNC45A antibody 19564-1-AP Download protocol IP protocol for UNC45A antibody 19564-1-AP Download protocol WB protocol for UNC45A antibody 19564-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- UNC-45A Is Highly Expressed in the Proliferative Cells of the Mouse Genital Tract and in the Microtubule-Rich Areas of the Mouse Nervous System.
Journal: Cells | Authors: Valentino Clemente | Species: mouse | Application: IHC,IF - A myosin chaperone, UNC-45A, is a novel regulator of intestinal epithelial barrier integrity and repair.
Journal: FASEB J | Authors: Susana Lechuga KO Validated | Species: human | Application: WB - UNC-45A breaks the microtubule lattice independently of its effects on non-muscle myosin II.
Journal: J Cell Sci | Authors: Juri Habicht | Species: rat | Application: WB,IF
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