USF1 Polyclonal antibody 22327-1-AP proteintech
$149.00
In stock
SKU
22327-1-AP
| Catalog Number | 22327-1-AP |
| Synonyms | bHLHb11, FCHL, FCHL1, HYPLIP1, MLTF, MLTFI, UEF, Upstream stimulatory factor 1, USF, USF1 |
| Host | Rabbit |
| Reactivity | human and More (1) |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ChIP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, K-562 cells, Jurkat cells |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Positive WB detected in | HeLa cells, K-562 cells, Jurkat cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag17789 Product name: Recombinant human USF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC035505 Sequence: MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGTGAISGYPATQSMTQAVIQGAFTSDDAVDTEGTAAETHYTYFPSTAVGDGAGGTTSGSTAAVVTTQGSEALLGQATPPGTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN Predict reactive species |
| Full Name | upstream transcription factor 1 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC035505 |
| Gene Symbol | USF1 |
| Gene ID (NCBI) | 7391 |
| RRID | AB_2879073 |
| Conjugate | Unconjugated |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P22415 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for USF1 antibody 22327-1-AP | Download protocol |
| IHC protocol for USF1 antibody 22327-1-AP | Download protocol |
| WB protocol for USF1 antibody 22327-1-AP | Download protocol |
| human | WB Dig Dis Sci WISP2 Overexpression Is Associated with Reduced Vasculogenic Mimicry and Epithelial-Mesenchymal Transition in Gastric Cancer. Authors - Xinning Xu View Article |
| human,mouse | WB J Oral Pathol Med LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription. Authors - Xiuhe Zou KD Validated View Article |
| Citations | 11 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:500-1:2000 IF/ICC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Upstream stimulatory factor 1 (USF1),also name Class B basic helix-loop-helix protein 11. it is a major late transcription factor 1.USF1 is a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E box motifs. USF1 has been linked to familial combined hyperlipidemia (FCHL). The USF1 exists two isoform and the calculated molecular weight of USF1 is a 32 kDa and 27 kDa. USF1 has acetylated form and phosphorylated form, so the molecular weight of USF1 can migrate to about 40 kDa Protocols Product Specific Protocols IF protocol for USF1 antibody 22327-1-AP Download protocol IHC protocol for USF1 antibody 22327-1-AP Download protocol WB protocol for USF1 antibody 22327-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Acute depletion of CTCF rewires genome-wide chromatin accessibility.
Journal: Genome Biol | Authors: Beisi Xu KO Validated | Species: human | Application: WB - Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen.
Journal: Elife | Authors: Hao Zhang KO Validated | Species: human | Application: WB - Upstream transcription factor 1 suppresses laryngeal squamous cell carcinoma progression through transcriptional activation of junctional adhesion molecule 3
Journal: IUBMB Life | Authors: Yue Jia | Species: human | Application: WB,IHC - LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.
Journal: J Oral Pathol Med | Authors: Xiuhe Zou KD Validated | Species: human,mouse | Application: WB - USF1-activated hsa_circ_0076691 induces oxaliplatin resistance via facilitating FGF9 expression in miR-589-3p-dependent manners
Journal: Noncoding RNA Res | Authors: Lingyu Tang | Species: human | Application: WB - WISP2 Overexpression Is Associated with Reduced Vasculogenic Mimicry and Epithelial-Mesenchymal Transition in Gastric Cancer.
Journal: Dig Dis Sci | Authors: Xinning Xu | Species: human | Application: WB - LncRNA MIR4435-2HG-mediated succinylation of USF1 promotes its protein stability and induces epithelial-mesenchymal transition in HNSCC.
Journal: Epigenetics | Authors: Shujin He | Species: human | Application: WB - Upstream transcription factor 1 suppresses laryngeal squamous cell carcinoma progression through transcriptional activation of junctional adhesion molecule 3
Journal: IUBMB Life | Authors: Yue Jia | Species: human | Application: WB,IHC - WISP2 Overexpression Is Associated with Reduced Vasculogenic Mimicry and Epithelial-Mesenchymal Transition in Gastric Cancer.
Journal: Dig Dis Sci | Authors: Xinning Xu | Species: human | Application: WB - LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.
Journal: J Oral Pathol Med | Authors: Xiuhe Zou | Species: human,mouse | Application: WB - Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen.
Journal: Elife | Authors: Hao Zhang | Species: human | Application: WB
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