USP35 Polyclonal antibody 24559-1-AP proteintech
$149.00
In stock
SKU
24559-1-AP
| Catalog Number | 24559-1-AP |
| Synonyms | EC:3.4.19.12, KIAA1372, Ubiquitin carboxyl-terminal hydrolase 35, Ubiquitin-specific-processing protease 35, USP34 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, CoIP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, HeLa cells, RAW 264.7 cells |
| Tested Applications — Positive IHC detected in | human colon cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:3000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Positive WB detected in | A549 cells, HeLa cells, RAW 264.7 cells |
| Positive IHC detected in | human colon cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag20002 Product name: Recombinant human USP35 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-342 aa of BC131489 Sequence: MPGLRRRASWRVSIPGSWPSQTRARLVSSTWATHAMSTASFRPYSWRLSSFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSE Predict reactive species |
| Full Name | ubiquitin specific peptidase 35 |
| Calculated Molecular Weight | 1018 aa, 113 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC131489 |
| Gene Symbol | USP35 |
| Gene ID (NCBI) | 57558 |
| RRID | AB_2879608 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for USP35 antibody 24559-1-AP | Download protocol |
| WB protocol for USP35 antibody 24559-1-AP | Download protocol |
| human | WB Transl Oncol USP35 promotes hepatocellular carcinoma proliferation through GASC1-mediated ROCK2 upregulation Authors - Gen Chen KD Validated View Article |
| mouse | WB Cell Death Discov Deubiquitinase USP35 regulates MDM4 degradation to promote endothelial ferroptosis and renal injury progression. Authors - Chenyang Han View Article |
| Citations | 9 |
| Dilutions | WB : 1:500-1:3000 IHC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999). Protocols Product Specific Protocols IHC protocol for USP35 antibody 24559-1-AP Download protocol WB protocol for USP35 antibody 24559-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The deubiquitinating enzyme USP35 regulates the stability of NRF2 protein
Journal: Open Life Sci | Authors: Dian Zhang | Species: human | Application: WB - Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis
Journal: Cell Death Discov | Authors: Guowen Lin | Species: human | Application: WB,IHC,CoIP - Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1
Journal: Commun Biol | Authors: Shuo Wang KD Validated | Species: human | Application: WB - USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer
Journal: Oncogenesis | Authors: Yi Xiao | Species: human - USP35 promotes hepatocellular carcinoma proliferation through GASC1-mediated ROCK2 upregulation
Journal: Transl Oncol | Authors: Gen Chen KD Validated | Species: human | Application: WB - Deubiquitinase USP35 regulates MDM4 degradation to promote endothelial ferroptosis and renal injury progression.
Journal: Cell Death Discov | Authors: Chenyang Han | Species: mouse | Application: WB - USP35 promotes hepatocellular carcinoma proliferation through GASC1-mediated ROCK2 upregulation
Journal: Transl Oncol | Authors: Gen Chen | Species: human | Application: WB - Regulation of survivin protein stability by USP35 is evolutionarily conserved
Journal: Biochem Biophys Res Commun | Authors: Wei Wang | Species: human | Application: WB - The deubiquitinating enzyme USP35 regulates the stability of NRF2 protein
Journal: Open Life Sci | Authors: Dian Zhang | Species: human | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
