USP37 Polyclonal antibody 18465-1-AP proteintech
$149.00
In stock
SKU
18465-1-AP
| Catalog Number | 18465-1-AP |
| Synonyms | EC:3.4.19.12, KIAA1594, Ubiquitin carboxyl-terminal hydrolase 37, Ubiquitin-specific-processing protease 37 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, mouse spleen tissue, rat brain tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | mouse brain tissue, mouse spleen tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag12513 Product name: Recombinant human USP37 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC112901 Sequence: MLTLQDNSFLSIDKVPSKDAEEMRLFLDAVHQNRLPAAMKPSQGSGSFGAILGSRTSQKETSRQLSYSDNQASAKRGSLETKDDIPFRKVLGNPGRGSIKTVAGSGIARTIPSLTSTSTPLRSGLLENRTEKRKRMISTGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSLKQSEENRTSGLLPLQSSSFYGSRAGSKEHSSGGTNLDRTNVSSQTPSAKRSLGFLPQPVPLSVKKLRCNQDYTGWNKPRVPLSSHQQQQLQGFSNLGNTCYMNAILQSLFSLQSFANDLLKQGIPWKKIPLNALIRRFAHLLVKKDICNSETKKDLLKKVKNAISATAERFSG Predict reactive species |
| Full Name | ubiquitin specific peptidase 37 |
| Calculated Molecular Weight | 885 aa, 100 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC112901 |
| Gene Symbol | USP37 |
| Gene ID (NCBI) | 57695 |
| RRID | AB_10598483 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86T82 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for USP37 antibody 18465-1-AP | Download protocol |
| IP protocol for USP37 antibody 18465-1-AP | Download protocol |
| WB protocol for USP37 antibody 18465-1-AP | Download protocol |
| human | WB Cancer Lett POH1 induces Smad3 deubiquitination and promotes lung cancer metastasis Authors - Yang Yuan View Article |
| mouse | WB Nucleic Acids Res Topoisomerase IIA in adult NSCs regulates SVZ neurogenesis by transcriptional activation of Usp37 Authors - Shangyao Qin View Article |
| Human | IHC Cell Death Dis USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer Authors - Sheng Li View Article |
| Citations | 17 |
| Dilutions | WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
USP37 (ubiquitin specific peptidase 37), is a member of ubiquitin-specific processing proteases family. Human USP37, localized mainly in the cytoplasm, is composed of 979 amino acids harboring three ubiquitin-interacting motifs between the Cys box and His box of the primary sequence (PMID: 27045719, 24324262). High levels of USP37 gene expression in lung cancer promoted cell viability as well as the Warburg effect via deubiquitination and stabilization of pluripotent factor c-Myc protein (PMID: 25284584). Moreover, USP37 expression was upregulated in breast cancer tissues compared with surrounding tissues and its overexpression was significantly correlated with increased rates of mortality. USP37 may represent a novel molecular target for breast cancer treatment (PMID: 30482232, 27045719). Protocols Product Specific Protocols IHC protocol for USP37 antibody 18465-1-AP Download protocol IP protocol for USP37 antibody 18465-1-AP Download protocol WB protocol for USP37 antibody 18465-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM.
Journal: Nucleic Acids Res | Authors: Chenming Wu KD Validated | Species: human | Application: WB,IHC,IF - Topoisomerase IIA in adult NSCs regulates SVZ neurogenesis by transcriptional activation of Usp37
Journal: Nucleic Acids Res | Authors: Shangyao Qin | Species: mouse | Application: WB - PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1.
Journal: Theranostics | Authors: Liang Wu KD Validated | Species: human | Application: IF - PMID: 30482232
Journal: J Exp Clin Cancer Res | Authors: Tao Qin KD Validated | Species: human | Application: WB,IHC,IF - USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer
Journal: Cell Death Dis | Authors: Sheng Li | Species: Human | Application: IHC - POH1 induces Smad3 deubiquitination and promotes lung cancer metastasis
Journal: Cancer Lett | Authors: Yang Yuan | Species: human | Application: WB - CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation.
Journal: Proc Natl Acad Sci U S A | Authors: Yunfan Yang | Species: human | Application: WB - Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.
Journal: Front Immunol | Authors: Wenying Gao | Species: human | Application: WB - Cyperotundone promotes chemosensitivity of breast cancer via SRSF1
Journal: Front Pharmacol | Authors: Chao Li | Species: human | Application: WB - An integrative pan-cancer analysis of USP37 and functional validation in pancreatic cancer
Journal: Front Cell Dev Biol | Authors: Jiafei Chen - Stabilization of estrogen receptor α by USP37 contributes to the progression of breast cancer
Journal: Cancer Sci | Authors: Jing Cao | Species: mouse,human | Application: WB,IP,IHC,IF - miR-4487 Enhances Gefitinib-Mediated Ubiquitination and Autophagic Degradation of EGFR in Non-Small Cell Lung Cancer Cells by Targeting USP37
Journal: Cancer Res Treat | Authors: Mi Seong Kim | Species: human | Application: WB - Deubiquitinase USP37 enhances the anti-HIV-2/SIV ability of the host restriction factor SAMHD1
Journal: J Virol | Authors: Wenzhe Cui | Species: human | Application: WB - USP37 downregulation elevates the Chemical Sensitivity of Human Breast Cancer Cells to Adriamycin.
Journal: Int J Med Sci | Authors: Tao Qin | Species: human | Application: WB,IHC - USP37 Promotes Lung Cancer Cell Migration by Stabilizing Snail Protein via Deubiquitination.
Journal: Front Genet | Authors: Jiali Cai | Species: human | Application: WB - The HBx Oncoprotein of Hepatitis B Virus Deregulates the Cell Cycle by Promoting the Intracellular Accumulation and Re-Compartmentalization of the Cellular Deubiquitinase USP37.
Journal: PLoS One | Authors: Nehul Saxena | Species: human | Application: WB,IP - Identification of Raptor and GLI1 as USP37 substrates highlight its context-specific function in medulloblastoma cells.
Journal: Oncogene | Authors: Ashutosh Singh
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