VAC14 Polyclonal antibody 15771-1-AP proteintech
$149.00
In stock
SKU
15771-1-AP
| Catalog Number | 15771-1-AP |
| Synonyms | Tax1-binding protein 2, TAX1BP2, TRX |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IP, CoIP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | human spleen tissue, Jurkat cells |
| Tested Applications — Positive IP detected in | Jurkat cells |
| Tested Applications — Positive IHC detected in | human skin tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Positive WB detected in | human spleen tissue, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human skin tissue, human lung tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8415 Product name: Recombinant human VAC14 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 228-576 aa of BC007214 Sequence: KMFRHTDSLFPILLQTLSDESDEVILKDLEVLAEIASSPAGQTDDPGPLDGPDLQASHSELQVPTPGRAGLLNTSGTKGLECSPSTPTMNSYFYKFMINLLKRFSSERKLLEVRGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHALNTILLTSTELFQLRNQLKDLKTLESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQKFGDLEVTVDFLAEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGLLMLLPQSSAFQLLSHRLQCVPNPELLQTEDSLKAAPKSQKADSPSIDYAELLQHFEKVQNKHLEVRHQRSGRGDHLDRRVVL Predict reactive species |
| Full Name | Vac14 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 88 kDa, 65 kDa |
| GenBank Accession Number | BC007214 |
| Gene Symbol | VAC14 |
| Gene ID (NCBI) | 55697 |
| RRID | AB_2214753 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08AM6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for VAC14 antibody 15771-1-AP | Download protocol |
| IP protocol for VAC14 antibody 15771-1-AP | Download protocol |
| WB protocol for VAC14 antibody 15771-1-AP | Download protocol |
| mouse | WB Exp Cell Res Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages. Authors - Shin Morioka KD Validated View Article |
| human | WB PLoS Biol The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model. Authors - Zhihui Zhou View Article |
| Citations | 4 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages.
Journal: Exp Cell Res | Authors: Shin Morioka KD Validated | Species: mouse | Application: WB - Ebola virus requires phosphatidylinositol (3,5) bisphosphate production for efficient viral entry.
Journal: Virology | Authors: Shirley Qiu KO Validated | Application: WB - Coxiella burnetii effector CvpE maintains biogenesis of Coxiella-containing vacuoles by suppressing lysosome tubulation through binding PI(3)P and perturbing PIKfyve activity on lysosomes
Journal: Virulence | Authors: Mingliang Zhao | Species: human | Application: CoIP - The lipid-metabolic enzyme HSD17B12 drives lysosomal degradation of PD-L1 potentiating anti-tumor immunity in a mouse model.
Journal: PLoS Biol | Authors: Zhihui Zhou | Species: human | Application: WB
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