| Catalog Number | CL488-85400-4 |
| Synonyms | VAMP-8, 242830B11, EDB, Endobrevin, VAMP 8 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag7903 Product name: Recombinant human VAMP8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-81 aa of BC001634 Sequence: MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLIC Predict reactive species |
| Full Name | vesicle-associated membrane protein 8 (endobrevin) |
| Calculated Molecular Weight | 11 kDa |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC001634 |
| Gene Symbol | VAMP8 |
| Gene ID (NCBI) | 8673 |
| RRID | AB_3747758 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BV40 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL Plus 488 VAMP8 antibody CL488-85400-4 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242830B11 |
| Conjugation | CoraLite® Plus 488 |
VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Protocols Product Specific Protocols IF protocol for CL Plus 488 VAMP8 antibody CL488-85400-4 Download protocol Standard Protocols Click here to view our Standard Protocols
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