VCAM-1/CD106 Polyclonal antibody 33377-1-AP proteintech
$149.00
In stock
SKU
33377-1-AP
| Catalog Number | 33377-1-AP |
| Synonyms | CD106, Soluble Vascular Cell Adhesion Molecule-1, Vascular cell adhesion protein 1, V-CAM 1, Vcam1 |
| Host | Rabbit |
| Reactivity | mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse spleen tissue, rat brain tissue, rat kidney tissue, rat spleen tissue |
| Tested Applications — Positive IHC detected in | rat spleen tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Positive WB detected in | mouse spleen tissue, rat brain tissue, rat kidney tissue, rat spleen tissue |
| Positive IHC detected in | rat spleen tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Tested Reactivity | mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Eg3821 Product name: Recombinant Rat VCAM-1/CD106 protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 25-698 aa of NM_012889.1 Sequence: FKIEISPEYKTLAQIGDSMLLTCSTTGCESPSFSWRTQIDSPLNGKVKTEGAKSVLTMDPVSFENEHSYLCTATCNSGKLERGIQVDIYSFPKDPEIQFSGPLEVGKPVMVKCLAPDVYPIDRLEIELFKGDRLMKKQDFVDEMAKKSLETKSLEVIFTPVIEDIEKALVCRAKLYIDQTDSIPKERETVRELQVYTSPKNTEISVHPSTRLHEGAAVTMTCASEGLPAPEIFWSKKLDNGVLQLLSGNATLTLIAMRMEDSGIYVCEGVNLVGRDKTEVELIVQEKPFTVDISPGSQVAAQVGDSVVLTCAAVGCDSPSFSWRTQTDSPLNGEVRDEGATSTLTLSPVGVEDEHSYLCTVTCQRRKLEKTIQVEVYSFPEDPEIEISGPLVHGRPVTVNCTVPNVYPFDHLEIELLKGETTLLNKFLREEIGTKSLETKSLEMTFIPTAEDTGKALVCLAKLHSSQMESEPKQRQSTQTLYVNVAPKEPTIWVSPSPVPEEGSPVNLTCSSDGFPTPKILWSRQLKNGELQPLSQNTTLSFMATKMEDSGIYVCEGINEAGISKKSVELIIQGSSKDIQLTVFPSKSVKEGDTVIISCTCGSVPEIWIILKKKAKTGDMVLKSVNGSYTIRKAQLQDAGVYECESKTEVGSQLRSLTLDVKGKENNKDYFSPE Predict reactive species |
| Full Name | vascular cell adhesion molecule 1 |
| Calculated Molecular Weight | 81 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | NM_012889.1 |
| Gene Symbol | Vcam1 |
| Gene ID (NCBI) | 25361 |
| RRID | AB_3742955 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P29534 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for VCAM-1/CD106 antibody 33377-1-AP | Download protocol |
| WB protocol for VCAM-1/CD106 antibody 33377-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843). Protocols Product Specific Protocols IHC protocol for VCAM-1/CD106 antibody 33377-1-AP Download protocol WB protocol for VCAM-1/CD106 antibody 33377-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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