VPS28 Polyclonal antibody 15478-1-AP proteintech
$149.00
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SKU
15478-1-AP
| Catalog Number | 15478-1-AP |
| Synonyms | ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting-associated protein 28 homolog |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Jurkat cells, HeLa cells, mouse brain tissue, rat brain tissue |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | Jurkat cells, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag7784 Product name: Recombinant human VPS28 protein Source: e coli. -derived, PKG Tag: GST Domain: 1-221 aa of BC006485 Sequence: MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA Predict reactive species |
| Full Name | vacuolar protein sorting 28 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 26-28 kDa |
| GenBank Accession Number | BC006485 |
| Gene Symbol | VPS28 |
| Gene ID (NCBI) | 51160 |
| RRID | AB_2878144 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK41 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for VPS28 antibody 15478-1-AP | Download protocol |
| IHC protocol for VPS28 antibody 15478-1-AP | Download protocol |
| IP protocol for VPS28 antibody 15478-1-AP | Download protocol |
| WB protocol for VPS28 antibody 15478-1-AP | Download protocol |
| human | WB J Cell Biol ESCRT-I and PTPN23 mediate microautophagy of ubiquitylated tau aggregates. Authors - Yusen Men View Article |
| Citations | 6 |
| Dilutions | WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:200-1:800 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described. Protocols Product Specific Protocols IF protocol for VPS28 antibody 15478-1-AP Download protocol IHC protocol for VPS28 antibody 15478-1-AP Download protocol IP protocol for VPS28 antibody 15478-1-AP Download protocol WB protocol for VPS28 antibody 15478-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.
Journal: PLoS Genet | Authors: Chiara Merigliano | Species: human | Application: IF - VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.
Journal: iScience | Authors: Xiaohua Dong KD Validated | Species: human | Application: WB,IF - Budget-Friendly Generation, Biochemical Analyses, and Lentiviral Transduction of Patient-Derived Colon Organoids
Journal: Curr Protoc | Authors: Emilie Rigaux | Species: human | Application: WB - Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy
Journal: Nat Commun | Authors: Takashi Nozawa KD Validated | Species: human | Application: WB,IF - RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer
Journal: J Extracell Biol | Authors: Yu-Chan Chang | Species: human | Application: WB - ESCRT-I and PTPN23 mediate microautophagy of ubiquitylated tau aggregates.
Journal: J Cell Biol | Authors: Yusen Men | Species: human | Application: WB
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