VPS4B Monoclonal antibody 68443-1-Ig proteintech
$149.00
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SKU
68443-1-Ig
| Catalog Number | 68443-1-Ig |
| Synonyms | VPS4A/B, 2H3G7, Cell migration-inducing gene 1 protein, EC:3.6.4.6, MIG1 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, IP, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | HuH-7 cells, hTERT-RPE1 cells, U-118 MG cells, HaCaT cells, MDA-MB-468 cells, RT-4 cells, RAW 264.7 cells, PC-12 cells, pig liver tissue |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | HuH-7 cells, hTERT-RPE1 cells, U-118 MG cells, HaCaT cells, MDA-MB-468 cells, RT-4 cells, RAW 264.7 cells, PC-12 cells, pig liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag11610 Product name: Recombinant human VPS4B protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-444 aa of BC039574 Sequence: MSSTSPNLQKAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAEKLKEYLKNKEKKAQKPVKEGQPSPADEKGNDSDGEGESDDPEKKKLQNQLQGAIVIERPNVKWSDVAGLEGAKEALKEAVILPIKFPHLFTGKRTPWRGILLFGPPGTGKSYLAKAVATEANNSTFFSISSSDLVSKWLGESEKLVKNLFQLARENKPSIIFIDEIDSLCGSRSENESEAARRIKTEFLVQMQGVGVDNDGILVLGATNIPWVLDSAIRRRFEKRIYIPLPEPHARAAMFKLHLGTTQNSLTEADFRELGRKTDGYSGADISIIVRDALMQPVRKVQSATHFKKVRGPSRADPNHLVDDLLTPCSPGDPGAIEMTWMDVPGDKLLEPVVSMSDMLRSLSNTKPTVNEHDLLKLKKFTEDFGQEG Predict reactive species |
| Full Name | vacuolar protein sorting 4 homolog B (S. cerevisiae) |
| Calculated Molecular Weight | 444 aa, 49 kDa |
| Observed Molecular Weight | ~50 kDa |
| GenBank Accession Number | BC039574 |
| Gene Symbol | VPS4B |
| Gene ID (NCBI) | 9525 |
| RRID | AB_3085156 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | O75351 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for VPS4B antibody 68443-1-Ig | Download protocol |
| IP protocol for VPS4B antibody 68443-1-Ig | Download protocol |
| WB protocol for VPS4B antibody 68443-1-Ig | Download protocol |
| human, mouse | WB Nat Commun Negative feedback regulation of STING signaling by TAX1BP1-directed Golgiphagy. Authors - Sujit Suklabaidya View Article |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:500-1:2000 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 2H3G7 |
| Conjugation | Unconjugated |
VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. (PMID:31930723) Protocols Product Specific Protocols IF protocol for VPS4B antibody 68443-1-Ig Download protocol IP protocol for VPS4B antibody 68443-1-Ig Download protocol WB protocol for VPS4B antibody 68443-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Negative feedback regulation of STING signaling by TAX1BP1-directed Golgiphagy.
Journal: Nat Commun | Authors: Sujit Suklabaidya | Species: human, mouse | Application: WB
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