ValRS Monoclonal antibody 67935-1-Ig proteintech
$149.00
In stock
SKU
67935-1-Ig
| Catalog Number | 67935-1-Ig |
| Synonyms | Valyl-tRNA synthetase, VARS, 1A2C3, EC:6.1.1.9, G7A |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG2a |
| Tested Applications — Positive WB detected in | HEK-293 cells, HepG2 cells, LNCaP cells, MCF-7 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Tested Applications — Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Positive WB detected in | HEK-293 cells, HepG2 cells, LNCaP cells, MCF-7 cells, HeLa cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Tested Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag9312 Product name: Recombinant human VARS protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 916-1264 aa of BC012808 Sequence: ECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHESLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLYTCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRSLRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQLQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALFQKML Predict reactive species |
| Full Name | valyl-tRNA synthetase |
| Calculated Molecular Weight | 1264 aa, 140 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC012808 |
| Gene Symbol | ValRS |
| Gene ID (NCBI) | 7407 |
| RRID | AB_2918687 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P26640 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ValRS antibody 67935-1-Ig | Download protocol |
| IHC protocol for ValRS antibody 67935-1-Ig | Download protocol |
| WB protocol for ValRS antibody 67935-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:250-1:1000 IF/ICC : 1:400-1:1600 |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Clone Number | 1A2C3 |
| Conjugation | Unconjugated |
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