WBP4 Polyclonal antibody 32283-1-AP proteintech
$149.00
In stock
SKU
32283-1-AP
| Catalog Number | 32283-1-AP |
| Synonyms | FBP21, FNBP21, Formin-binding protein 21, WBP-4, WW domain-binding protein 4 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells |
| Tested Applications — Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U-251 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:5000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag35400 Product name: Recombinant human WBP4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC108310 Sequence: MADYWKSQPKKFCDYCKCWIADNRPSVEFHERGKNHKENVAKRISEIKQKSLDKAKEEEKASKEFAAMEAAALKAYQEDLKRLGLESEILEPSITPVTSTIPPTSTSNQQKEKKEKKKRKKDPSKGRWVEGITSEGYHYYYDLISGASQW Predict reactive species |
| Full Name | WW domain binding protein 4 (formin binding protein 21) |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC108310 |
| Gene Symbol | WBP4 |
| Gene ID (NCBI) | 11193 |
| RRID | AB_3742568 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O75554 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for WBP4 antibody 32283-1-AP | Download protocol |
| IHC protocol for WBP4 antibody 32283-1-AP | Download protocol |
| WB protocol for WBP4 antibody 32283-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:5000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
WW domain-binding protein 4 (WBP4), formerly known as FBP21, is a 376-amino acid spliceosomal protein containing a zinc finger motif and two tandem WW domains. It is a member of the WW domain-containing protein family, which plays a role in various cellular processes including signal transduction, transcriptional regulation and protein degradation. The protein is characterised by its WW domains, which mediate interactions with proline-rich motifs in other proteins. WBP4 is involved in key signalling pathways such as Wnt and Notch and interacts with transcription factors and components of the ubiquitin-proteasome system. Protocols Product Specific Protocols IF protocol for WBP4 antibody 32283-1-AP Download protocol IHC protocol for WBP4 antibody 32283-1-AP Download protocol WB protocol for WBP4 antibody 32283-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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