| Catalog Number | 23404-1-AP |
| Synonyms | C19orf14, WD repeat domain 62, WD repeat-containing protein 62 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IP detected in | HEK-293T cells |
| Tested Applications — Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | hTERT-RPE1 cells, A549 cells |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | hTERT-RPE1 cells, A549 cells |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag19865 Product name: Recombinant human WDR62 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 281-630 aa of BC017261 Sequence: CLTSLASCVPASSVLPTDRNLPTPTSAPTPGLAQGVHAPSTCSYMEATASSRARISRSISLGDSEGPIVATLAQPLRRPSSVGELASLGQELQAITTATTPSLDSEGQEPALRSWGNHEARANLRLTLSSACDGLLLPPVDTQPGVTVPAVSFPAPSPVEESALRLHGSAFRPSLPAPESPGLPAHPSNPQLPEARPGIPGSTASLLEPTSGALGLFQGSPARWSEPWVPVEALPPSPLELSRVGNILHRLQTTFQEALDLYRVLVSSGQVDTGQQQARTELVSTFLWIHSQLEAECLVGTSVAPAQALPSPGPPSPPTLYPLASPDLQALLEHYSELLVQAVRRKARGH Predict reactive species |
| Full Name | WD repeat domain 62 |
| Calculated Molecular Weight | 1518 aa, 166 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC017261 |
| Gene Symbol | WDR62 |
| Gene ID (NCBI) | 284403 |
| RRID | AB_3085711 |
| Conjugate | Unconjugated |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | O43379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for WDR62 antibody 23404-1-AP | Download protocol |
| IHC protocol for WDR62 antibody 23404-1-AP | Download protocol |
| IP protocol for WDR62 antibody 23404-1-AP | Download protocol |
| Citations | - |
| Dilutions | IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
The WD40-repeat protein 62 (WDR62) is a scaffold protein that recruits different components of the JNK signaling pathway to regulate several human diseases including neurological disorders, infertility, and tumorigenesis (PMID: 33937237). Moreover, WDR62 participates in diverse biological processes, especially mitotic spindle organization via regulating centriole biogenesis and the function of centriole-associated protein (PMID: 35093571). Protocols Product Specific Protocols IF protocol for WDR62 antibody 23404-1-AP Download protocol IHC protocol for WDR62 antibody 23404-1-AP Download protocol IP protocol for WDR62 antibody 23404-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols