XPNPEP3 Polyclonal antibody 15655-1-AP proteintech
$149.00
In stock
SKU
15655-1-AP
| Catalog Number | 15655-1-AP |
| Synonyms | Aminopeptidase P3, APP3, APP3c, APP3m, EC:3.4.11.9 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A431 cells, human testis tissue, human spleen tissue, PC-3 cells, 4T1 cells, mouse liver |
| Tested Applications — Positive IP detected in | PC-3 cells |
| Tested Applications — Positive IHC detected in | mouse liver tissue, human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:8000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Positive WB detected in | A431 cells, human testis tissue, human spleen tissue, PC-3 cells, 4T1 cells, mouse liver |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse liver tissue, human oesophagus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8007 Product name: Recombinant human XPNPEP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 158-507 aa of BC004989 Sequence: PSRELWDGPRSGTDGAIALTGVDEAYTLEEFQHLLPKMKAETNMVWYDWMRPSHAQLHSDYMQPLTEAKAKSKNKVRGVQQLIQRLRLIKSPAEIERMQIAGKLTSQAFIETMFTSKAPVEEAFLYAKFEFECRARGADILAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQAELYEAVLEIQRDCLALCFPGTSLENIYSMMLTLIGQKLKDLGIMKNIKENNAFKAARKYCPHHVGHYLGMDVHDTPDMPRSLPLQPGMVITIEPGIYIPEDDKDAPEKFRGLGVRIEDDVVVTQDSPLILSADCPKEMNDIEQICSQAS Predict reactive species |
| Full Name | X-prolyl aminopeptidase (aminopeptidase P) 3, putative |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC004989 |
| Gene Symbol | XPNPEP3 |
| Gene ID (NCBI) | 63929 |
| RRID | AB_2288500 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQH7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for XPNPEP3 antibody 15655-1-AP | Download protocol |
| IHC protocol for XPNPEP3 antibody 15655-1-AP | Download protocol |
| IP protocol for XPNPEP3 antibody 15655-1-AP | Download protocol |
| WB protocol for XPNPEP3 antibody 15655-1-AP | Download protocol |
| Citations | - |
| Dilutions | WB : 1:1000-1:8000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF-P : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
XPNPEP3 gene encodes X-prolyl aminopeptidase 3 which functions to remove the penultimate N-terminal Proline residue from nascent proteins and appears to play a role in ciliary function. CRC tissue microarray revealed increased XPNPEP3 expression in tumor compared to matched normal samples (PMID:29383790). Other study found that XPNPEP3 localizes to mitochondria in renal cells in vitro and to kidney tubules in a cell type-specific pattern, and mutations in the gene are associated with NPHP-like disorder (PMID:20179356). Protocols Product Specific Protocols IF protocol for XPNPEP3 antibody 15655-1-AP Download protocol IHC protocol for XPNPEP3 antibody 15655-1-AP Download protocol IP protocol for XPNPEP3 antibody 15655-1-AP Download protocol WB protocol for XPNPEP3 antibody 15655-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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