YPEL5 Polyclonal antibody 11730-1-AP proteintech
$149.00
In stock
SKU
11730-1-AP
| Catalog Number | 11730-1-AP |
| Synonyms | CGI 127, Protein yippee like 5, yippee like 5 (Drosophila), YPEL5 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, zebrafish |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
| Tested Applications — Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2328 Product name: Recombinant human YPEL5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-121 aa of BC000836 Sequence: MGRIFLDHIGGTRLFSCANCDTILTNRSELISTRFTGATGRAFLFNKVVNLQYSEVQDRVMLTGRHMVRDVSCKNCNSKLGWIYEFATEDSQRYKEGRVILERALVRESEGFEEHVPSDNS Predict reactive species |
| Full Name | yippee-like 5 (Drosophila) |
| Calculated Molecular Weight | 121 aa, 14 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC000836 |
| Gene Symbol | YPEL5 |
| Gene ID (NCBI) | 51646 |
| RRID | AB_2217465 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62699 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for YPEL5 antibody 11730-1-AP | Download protocol |
| WB protocol for YPEL5 antibody 11730-1-AP | Download protocol |
| zebrafish | WB,IF Neuron Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning. Authors - Hilliard Austin T AT View Article |
| human | WB iScience Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24 Authors - Lalit R Patel View Article |
| mouse | WB Nat Commun Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth Authors - Nelson V Simwela View Article |
| Abigail (Verified Customer) (02-26-2026) | The signal is quite faint in low expressing cell lines, but there. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: HEK293T |
| S (Verified Customer) (11-22-2021) | The band was there on the right MW range, but it was a bit weak. It could have been better if I used 0.2 um pore-size membrane, given that YPEL5 is a small protein. Applications: Western Blot Primary Antibody Dilution: 1:2000 Cell Tissue Type: HEK293T YPEL5 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11730-1-AP) |
| Citations | 6 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
YPEL5(Protein yippee-like 5) belongs to the yippee family and is involved in a certain cell division-related function.During cell cycle, YPEL5 protein is detected at different subcellular localizations; at interphase, it is located in the nucleus and centrosome, then it changes location sequentially to spindle poles, mitotic spindle, and spindle midzone during mitosis, and finally transferrs to midbody at cytokinesis(PMID:20580816). Protocols Product Specific Protocols IHC protocol for YPEL5 antibody 11730-1-AP Download protocol WB protocol for YPEL5 antibody 11730-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning.
Journal: Neuron | Authors: Hilliard Austin T AT | Species: zebrafish | Application: WB,IF - METTL3/YTHDF2 m6A axis accelerates colorectal carcinogenesis through epigenetically suppressing YPEL5.
Journal: Mol Oncol | Authors: Dan Zhou | Species: human | Application: WB - Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth
Journal: bioRxiv | Authors: Nelson V Simwela | Species: human | Application: WB - Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesis.
Journal: bioRxiv | Authors: Drew W. Grant | Species: human | Application: WB,IP - Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24
Journal: iScience | Authors: Lalit R Patel | Species: human | Application: WB - Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth
Journal: Nat Commun | Authors: Nelson V Simwela | Species: mouse | Application: WB
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