YTHDF2 Polyclonal antibody 26771-1-AP proteintech
$149.00
In stock
SKU
26771-1-AP
| Catalog Number | 26771-1-AP |
| Synonyms | CLL associated antigen KW 14, CLL-associated antigen KW-14, DF2, HGRG8, NY REN 2 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Raji cells, Jurkat cells, mouse brain tissue |
| Tested Applications — Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | Raji cells, Jurkat cells, mouse brain tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag25348 Product name: Recombinant human YTHDF2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 289-348 aa of BC002559 Sequence: SQALVQNIGQPTQGSPQPVGQQANNSPPVAQASVGQQTQPLPPPPPQPAQLSVQQQAAQP Predict reactive species |
| Full Name | YTH domain family, member 2 |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC002559 |
| Gene Symbol | YTHDF2 |
| Gene ID (NCBI) | 51441 |
| RRID | AB_2880629 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5A9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for YTHDF2 antibody 26771-1-AP | Download protocol |
| IHC protocol for YTHDF2 antibody 26771-1-AP | Download protocol |
| WB protocol for YTHDF2 antibody 26771-1-AP | Download protocol |
| mouse | WB J Neurochem METTL14 is decreased and regulates m6 A modification of α-synuclein in Parkinson's disease Authors - Honghu He View Article |
| human | WB,IP,IF Mol Cell The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance Authors - Tao Liu KD Validated View Article |
| Yanhong (Verified Customer) (12-04-2018) | Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: HaCaT cells YTHDF2 Antibody Western Blot, validation (1:1000 dilution) in HaCaT cells (Cat no:26771-1-AP) |
| Citations | 3 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa. Protocols Product Specific Protocols IF protocol for YTHDF2 antibody 26771-1-AP Download protocol IHC protocol for YTHDF2 antibody 26771-1-AP Download protocol WB protocol for YTHDF2 antibody 26771-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- METTL14 is decreased and regulates m6 A modification of α-synuclein in Parkinson's disease
Journal: J Neurochem | Authors: Honghu He | Species: mouse | Application: WB - N6-Methyladenosine modification mediated by METTL3 promotes DNA-PKcs expression to induce anlotinib resistance in osteosarcoma
Journal: Clin Transl Med | Authors: Yining Zhang - The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance
Journal: Mol Cell | Authors: Tao Liu KD Validated | Species: human | Application: WB,IP,IF
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