Protrudin Polyclonal antibody 12680-1-AP proteintech
$149.00
In stock
SKU
12680-1-AP
| Catalog Number | 12680-1-AP |
| Synonyms | ZFYVE27, RP11 459F3.2, Spastic paraplegia 33 protein, SPG33, Zinc finger FYVE domain-containing protein 27 |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, monkey |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, CoIP, ELISA |
| Applications | WB, IHC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Positive WB detected in | mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3369 Product name: Recombinant human ZFYVE27 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 217-411 aa of BC030621 Sequence: VEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK Predict reactive species |
| Full Name | zinc finger, FYVE domain containing 27 |
| Calculated Molecular Weight | 411 aa, 46 kDa |
| Observed Molecular Weight | 46-50 kDa |
| GenBank Accession Number | BC030621 |
| Gene Symbol | ZFYVE27 |
| Gene ID (NCBI) | 118813 |
| RRID | AB_10640298 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5T4F4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for Protrudin antibody 12680-1-AP | Download protocol |
| IP protocol for Protrudin antibody 12680-1-AP | Download protocol |
| WB protocol for Protrudin antibody 12680-1-AP | Download protocol |
| human | WB Cell Mol Life Sci Protrudin regulates FAK activation, endothelial cell migration and angiogenesis. Authors - Amita Arora KD Validated View Article |
| rat | WB Nat Commun Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS. Authors - Veselina Petrova View Article |
| Citations | 16 |
| Dilutions | WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract. Protocols Product Specific Protocols IHC protocol for Protrudin antibody 12680-1-AP Download protocol IP protocol for Protrudin antibody 12680-1-AP Download protocol WB protocol for Protrudin antibody 12680-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria.
Journal: Nat Commun | Authors: Yael Elbaz-Alon | Species: human | Application: WB,IP - Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS.
Journal: Nat Commun | Authors: Veselina Petrova | Species: rat | Application: WB - Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.
Journal: Nat Commun | Authors: Parikshit Bagchi | Species: human | Application: WB - Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Journal: Proc Natl Acad Sci U S A | Authors: Chang Jaerak J KD Validated | Species: human | Application: WB,IP,IF - ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.
Journal: Cell Mol Life Sci | Authors: James W Connell | Species: human - Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.
Journal: Cell Mol Life Sci | Authors: Amita Arora KD Validated | Species: human | Application: WB - Protrudin modulates seizure activity through GABAA receptor regulation.
Journal: Cell Death Dis | Authors: Xi Lu | Species: mouse,human | Application: WB,IF,CoIP - Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Journal: Proc Natl Acad Sci U S A | Authors: Chang Jaerak J | Species: human | Application: WB,IP,IF - A kinesin 1-protrudin complex mediates AMPA receptor synaptic removal during long-term depression.
Journal: Cell Rep | Authors: Anna Brachet | Species: rat | Application: WB,IP - ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.
Journal: Cell Mol Life Sci | Authors: James W Connell | Species: human - Protrudin-mediated ER-endosome contact sites promote phagocytosis
Journal: Cell Mol Life Sci | Authors: Liv Anker Elfmark | Species: human | Application: IF - Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.
Journal: Cell Mol Life Sci | Authors: Amita Arora | Species: human | Application: WB - Protrudin acts at ER-endosome contacts to promote KIF5-mediated endosomal tubule fission.
Journal: Neurobiol Dis | Authors: Julia Kleniuk | Species: human | Application: WB - Transcription factor EB regulates phosphatidylinositol-3-phosphate levels that control lysosome positioning in the bladder cancer model
Journal: Commun Biol | Authors: Pallavi Mathur | Species: human | Application: WB,IF - Fatty acid synthase cooperates with protrudin to facilitate membrane outgrowth of cellular protrusions.
Journal: Sci Rep | Authors: Chuanling Zhang | Species: human | Application: WB - Protrudin Regulates Endoplasmic Reticulum Morphology and Function Associated with the Pathogenesis of Hereditary Spastic Paraplegia.
Journal: J Biol Chem | Authors: Yutaka Hashimoto | Species: human | Application: WB
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