ZNF618 Recombinant monoclonal antibody 84998-1-RR proteintech
$149.00
In stock
SKU
84998-1-RR
| Catalog Number | 84998-1-RR |
| Synonyms | 242566D9, KIAA1952, Zinc finger protein 618 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, SH-SY5Y cells, MCF-7 cells, Caki-2 cells |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A549 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | HEK-293 cells, SH-SY5Y cells, MCF-7 cells, Caki-2 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag36487 Product name: Recombinant human ZNF618 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 200-382 aa of NM_001318042.1 Sequence: YSCFQEHRDLHAVDVFSVEGAPENRADPFDQGVVATDEVKEEPPEPFQKIGPKTGNYTCEFCGKQYKYYTPYQEHVALHAPISTAPGWEPPDDPDTGSECSHPEVSPSPRFVAAKTQTNQSGKKAPASVVRCATLLHRTPPATQTQTFRTPNSGSPASKATAAESAFSRRVEGKAQNHFEETN Predict reactive species |
| Full Name | zinc finger protein 618 |
| Calculated Molecular Weight | 105kDa，954aa |
| Observed Molecular Weight | 110-130 kDa |
| GenBank Accession Number | NM_001318042.1 |
| Gene Symbol | ZNF618 |
| Gene ID (NCBI) | 114991 |
| RRID | AB_3743923 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5T7W0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ZNF618 antibody 84998-1-RR | Download protocol |
| IHC protocol for ZNF618 antibody 84998-1-RR | Download protocol |
| WB protocol for ZNF618 antibody 84998-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:250-1:1000 IF/ICC : 1:500-1:2000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242566D9 |
| Conjugation | Unconjugated |
Zinc finger protein 618 (ZNF618) is a key protein associated with DNA methylation and acts as a specific 5hmC reader in vivo to regulate UHRF2 function by promoting UHRF2 chromatin localization. In addition, ZNF618 is not only associated with the induction, maintenance or progression of female breast cancer, but is also up-regulated in lung cancer cells. Protocols Product Specific Protocols IF protocol for ZNF618 antibody 84998-1-RR Download protocol IHC protocol for ZNF618 antibody 84998-1-RR Download protocol WB protocol for ZNF618 antibody 84998-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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