CoraLite®555-conjugated Acetyl-Tubulin (Lys40) Monoclonal antibody CL555-66200 proteintech
$479.00
In stock
SKU
CL555-66200
| Catalog Number | CL555-66200 |
| Synonyms | Ac Tubulin (Lys40), Ac Tubulin Lys40, Acetyl Tubulin, Acetyl Tubulin (Lys40), Acetyl Tubulin Lys40 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig, canine |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive IF/ICC detected in | MDCK cells, hTERT-RPE1 cells |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive IF/ICC detected in | MDCK cells, hTERT-RPE1 cells |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat, pig, canine |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | tubulin, alpha 1a |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | NM_006009 |
| Gene Symbol | Alpha Tubulin |
| Gene ID (NCBI) | 7846 |
| RRID | AB_2919679 |
| Conjugate | CoraLite®555 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
| Excitation Laser | Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Purification Method | Protein G purification |
| UNIPROT ID | Q71U36 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| IF protocol for CL555 Acetyl-Tubulin (Lys40) antibody CL555-66200 | Download protocol |
| Citations | - |
| Dilutions | IF/ICC : 1:50-1:500 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 7E5H8 |
| Conjugation | CoraLite®555 |
What is the molecular weight of acetyl-α-tubulin? The molecular weight of acetylated tubulin is 52 kD. Where does the acetylation of Lys 40 occur? This acetylation occurs inside the microtubule lumen by the α-tubulin acetyltransferase 1 (αTAT1) (PMID: 29207274). How can acetylation be reversed? Acetylation of Lys 40 can be reversed by deacetylase 6 (HDAC6), which is mostly cytoplasmic that also deacetylates Hsp90, and sirtuin 2 (SIRT2), which also mainly cytoplasmic and uses NAD as a coenzyme. Unlike HDAC6, SIRT uses both polymerized and soluble tubulin as substrates. Deacetylases are believed to be more active on soluble tubulin, while acetylases function preferentially on stable polymers (PMIDs: 29207274, 30079247, 19185337). What is the function of acetylation? Acetylation is a conserved post-translational modification of alpha tubulin at Lys 40 during tubulin assembly, and it correlates to increased microtubule stability and intracellular transport (PMIDs: 29207274, 30079247, 20940043). Is acetylation of α-tubulin strictly associated with stable microtubules? Not necessarily, as acetylation can have other effects on microtubule subpopulations (PMID 20940043). Is ac-tubulin found only in cilia? Acetylated-α-tubulin is located in cytoplasmic tubulin as well as in cilia; therefore, it is not strictly region-specific (PMID: 30079247). What are the cellular effects of tubulin acetylation? Microtubule acetylation seems to provide a critical role in neuronal development and function, and while its effect on cancer cells remains unclear, it has been shown that decreased acetylated α-tubulin impairs neuronal cell line migration. The post-translational modification may also help regulate organelle-independent signalling throughout the cell, supporting the notion of a microtubule network serving as a coordinator of cellular signaling (PMIDs: 29207274, 25503560, 20940043, 19185337). Protocols Product Specific Protocols IF protocol for CL555 Acetyl-Tubulin (Lys40) antibody CL555-66200 Download protocol Standard Protocols Click here to view our Standard Protocols
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