Cardiac Troponin T Recombinant monoclonal antibody 81638-3-RR proteintech
$149.00
In stock
SKU
81638-3-RR
| Catalog Number | 81638-3-RR |
| Synonyms | cTnT, TNNT2, Troponin T, cardiac muscle |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse heart tissue, rat heart tissue, human heart tissue, pig heart tissue |
| Tested Applications — Positive IHC detected in | mouse heart tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse heart tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Positive WB detected in | mouse heart tissue, rat heart tissue, human heart tissue, pig heart tissue |
| Positive IHC detected in | mouse heart tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:250-1:1000 |
| Tested Reactivity | human, mouse, rat, pig |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag7716 Product name: Recombinant human cTnT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC002653 Sequence: MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK Predict reactive species |
| Full Name | troponin T type 2 (cardiac) |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 34-40 kDa |
| GenBank Accession Number | BC002653 |
| Gene Symbol | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_3743389 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | P45379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for Cardiac Troponin T antibody 81638-3-RR | Download protocol |
| IHC protocol for Cardiac Troponin T antibody 81638-3-RR | Download protocol |
| WB protocol for Cardiac Troponin T antibody 81638-3-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:1000-1:4000 IF-P : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250230B1 |
| Conjugation | Unconjugated |
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa. Protocols Product Specific Protocols IF protocol for Cardiac Troponin T antibody 81638-3-RR Download protocol IHC protocol for Cardiac Troponin T antibody 81638-3-RR Download protocol WB protocol for Cardiac Troponin T antibody 81638-3-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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