HEY2 Polyclonal antibody 10597-1-AP proteintech
$149.00
In stock
SKU
10597-1-AP
| Catalog Number | 10597-1-AP |
| Synonyms | bHLHb32, Cardiovascular helix-loop-helix factor 1, CHF1, Class B basic helix-loop-helix protein 32, GRIDLOCK |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, IP, CoIP, chIP, ELISA |
| Applications | WB, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | K-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells |
| Tested Applications — Positive IP detected in | mouse heart tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:1000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Positive WB detected in | K-562 cells, mouse heart tissue, MCF-7 cells, 4T1 cells |
| Positive IP detected in | mouse heart tissue |
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag0913 Product name: Recombinant human HEY2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-337 aa of BC007707 Sequence: MKRPCEETTSESDMDETIDVGSENNYSGQSTSSVIRLNSPTTTSQIMARKKRRGIIEKRRRDRINNSLSELRRLVPTAFEKQGSAKLEKAEILQMTVDHLKMLQATGGKGYFDAHALAMDFMSIGFRECLTEVARYLSSVEGLDSSDPLRVRLVSHLSTCATQREAAAMTSSMAHHHHPLHPHHWAAAFHHLPAALLQPNGLHASESTPCRLSTTSEVPPAHGSALLTATFAHADSALRMPSTGSVAPCVPPLSTSLLSLSATVHAAAAAATAAAHSFPLSFAGAFPMLPPNAAAAVAAATAISPPLSVSATSSPQQTSSGTNNKPYRPWGTEVGAF Predict reactive species |
| Full Name | hairy/enhancer-of-split related with YRPW motif 2 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 33-40 kDa |
| GenBank Accession Number | BC007707 |
| Gene Symbol | HEY2 |
| Gene ID (NCBI) | 23493 |
| RRID | AB_2118415 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBP5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for HEY2 antibody 10597-1-AP | Download protocol |
| IP protocol for HEY2 antibody 10597-1-AP | Download protocol |
| WB protocol for HEY2 antibody 10597-1-AP | Download protocol |
| human | WB Nat Commun Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia. Authors - Haiying Jiang View Article |
| mouse | WB Nat Neurosci Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination. Authors - Lai Man Natalie Wu View Article |
| CHAO (Verified Customer) (03-31-2022) | Weak band around correct size. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mouse Heart |
| Tom (Verified Customer) (08-22-2020) | This Hey2 antibody is highly recommended for detecting Hey2 on frozen tissue sections! I used this Hey2 antibody for immunofluorescence (IF). It clearly had nuclear staining signal in compact myocardium of embryonic heart, which is consistent with Hey2's well-known expression pattern. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: Mouse embryonic heart |
| Citations | 54 |
| Dilutions | WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Hairy/enhancer of split-related proteins, such as HEY2, are basic helix-loop-helix (bHLH) transcription factors implicated in cell fate decision and boundary formation. HEY genes are direct transcriptional targets of the Notch signaling pathways in Drosophila and vertebrates. HEY2 is similarly expressed in the somites whereas it shows a complementary expression in the heart, the craniofacial region and the nervous system. This antibody is a rabbit polyclonal antibody raised against full length of human HEY2. Protocols Product Specific Protocols IF protocol for HEY2 antibody 10597-1-AP Download protocol IP protocol for HEY2 antibody 10597-1-AP Download protocol WB protocol for HEY2 antibody 10597-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Multi-chamber cardioids unravel human heart development and cardiac defects
Journal: Cell | Authors: Clara Schmidt | Species: human | Application: IF - Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts.
Journal: Cell Stem Cell | Authors: Blair K Gage | Species: human | Application: IF - Zeb2 recruits HDAC-NuRD to inhibit Notch and controls Schwann cell differentiation and remyelination.
Journal: Nat Neurosci | Authors: Lai Man Natalie Wu | Species: mouse | Application: WB - Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis.
Journal: Nat Commun | Authors: Jared M Churko | Species: human | Application: chIP - Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.
Journal: Nat Commun | Authors: Shunsuke Funakoshi | Species: human | Application: IF - Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.
Journal: Nat Commun | Authors: Haiying Jiang | Species: human | Application: WB - The transcriptional repressor HEY2 regulates mitochondrial oxidative respiration to maintain cardiac homeostasis
Journal: Nat Commun | Authors: Peilu She | Application: WB - Lactate derived from macrophages drives skin dermal fibroblasts phenotypic remodeling via MCT1-primed histone H3 lysine 23 lactylation in hypertrophic scar
Journal: Nat Commun | Authors: Yixuan Yuan - Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells.
Journal: Nat Commun | Authors: Shunsuke Funakoshi | Species: human | Application: IF - Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia.
Journal: Nat Commun | Authors: Haiying Jiang | Species: human | Application: WB - Vasorin-containing small extracellular vesicles retard intervertebral disc degeneration utilizing an injectable thermoresponsive delivery system
Journal: J Nanobiotechnology | Authors: Zhiwei Liao | Species: human | Application: WB - MicroRNA-136 inhibits cancer stem cell activity and enhances the anti-tumor effect of paclitaxel against chemoresistant ovarian cancer cells by targeting Notch3.
Journal: Cancer Lett | Authors: Ju-Yeon Jeong | Species: human | Application: WB - Interaction between BEND5 and RBPJ suppresses breast cancer growth and metastasis via inhibiting Notch signaling.
Journal: Int J Biol Sci | Authors: Yanzhu Shi | Species: human | Application: WB,CoIP - TMEM100 is a key factor for specification of lymphatic endothelial progenitors.
Journal: Angiogenesis | Authors: Eun-Hye Moon | Species: mouse - DEHP exposure impairs mouse oocyte cyst breakdown and primordial follicle assembly through estrogen receptor-dependent and independent mechanisms.
Journal: J Hazard Mater | Authors: Xinyi Mu | Species: mouse | Application: WB - Pseudogene ACTBP2 increases blood-brain barrier permeability by promoting KHDRBS2 transcription through recruitment of KMT2D/WDR5 in Aβ1-42 microenvironment.
Journal: Cell Death Discov | Authors: Qianshuo Liu | Species: human | Application: WB,IF,ChIP - OGT mediated HDAC5 O-GlcNAcylation promotes osteogenesis by regulating the homeostasis of epigenetic modifications and proteolysis
Journal: J Orthop Translat | Authors: Yu Du | Species: mouse | Application: WB - Tmem100, an ALK1 receptor signaling-dependent gene essential for arterial endothelium differentiation and vascular morphogenesis.
Journal: Proc Natl Acad Sci U S A | Authors: Somekawa Satoshi S | Species: mouse | Application: IHC - Identification of signaling pathways that specify a subset of migrating enteric neural crest cells at the wavefront in mouse embryos
Journal: Dev Cell | Authors: Bingyan Zhou | Species: mouse | Application: IF - Villus myofibroblasts are developmental and adult progenitors of mammalian gut lymphatic musculature
Journal: Dev Cell | Authors: Bhargav D Sanketi - Rbpj conditional knockout reveals distinct functions of Notch4/Int3 in mammary gland development and tumorigenesis.
Journal: Oncogene | Authors: Raafat A A | Species: mouse | Application: IHC - MicroRNA-98 reduces amyloid β-protein production and improves oxidative stress and mitochondrial dysfunction through the Notch signaling pathway via HEY2 in Alzheimer's disease mice.
Journal: Int J Mol Med | Authors: Fang-Zhou Chen | Species: mouse | Application: WB,IHC - Pancreatic ductal deletion of Hnf1b disrupts exocrine homeostasis, leads to pancreatitis and facilitates tumorigenesis.
Journal: Cell Mol Gastroenterol Hepatol | Authors: Evans Quilichini | Species: mouse | Application: IHC - TAp73 acts via the bHLH Hey2 to promote long-term maintenance of neural precursors.
Journal: Curr Biol | Authors: Masashi Fujitani | Species: mouse | Application: WB - Genetic modifiers predisposing to congenital heart disease in the sensitized Down syndrome population.
Journal: Circ Cardiovasc Genet | Authors: Li Huiqing H | Species: mouse,human | Application: WB - Subtype-specific role for Jagged1 in promoting or inhibiting breast tumor formation
Journal: Oncogenesis | Authors: Wen-Cheng Chung | Species: mouse | Application: IHC - MEK/ERK signaling drives the transdifferentiation of supporting cells into functional hair cells by modulating the Notch pathway
Journal: Stem Cells Transl Med | Authors: Jiaoyao Ma | Species: mouse | Application: WB - Super-Enhancers and Mef2c: Novel Regulators of Cardiac Hypertrophy Via the Hey2/Notch/p38 Signaling Pathway
Journal: Eur J Pharmacol | Authors: Ruixuan Wang | Species: mouse | Application: WB - Notch Signaling Induced by Endoplasmic Reticulum Stress Regulates Cumulus-Oocyte Complex Expansion in Polycystic Ovary Syndrome
Journal: Biomolecules | Authors: Hiroshi Koike | Species: mouse,human | Application: IHC - JAG2/Notch2 inhibits intervertebral disc degeneration by modulating cell proliferation, apoptosis, and extracellular matrix.
Journal: Arthritis Res Ther | Authors: Jun Long | Application: WB - JAG2/Notch2 inhibits intervertebral disc degeneration by modulating cell proliferation, apoptosis, and extracellular matrix.
Journal: Arthritis Res Ther | Authors: Jun Long | Application: WB - Cigarette smoke enhances initiation and progression of lung cancer by mutating Notch1/2 and dysregulating downstream signaling molecules.
Journal: Oncotarget | Authors: Wei Li | Species: human | Application: WB - Extrahepatic cholangiocyte obstruction is mediated by decreased glutathione, Wnt and Notch signaling pathways in a toxic model of biliary atresia.
Journal: Sci Rep | Authors: Sophia Fried | Species: mouse,human | Application: IF - Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.
Journal: Cancer Sci | Authors: Guan-Zhong Qiu | Species: human | Application: WB - 2B.06: EPIGALLOCATECHIN-3-GALLATE ATTENUATES URIC ACID-INDUCED INFLAMMATORY RESPONSES AND OXIDATIVE STRESS BY MODULATING NOTCH PATHWAY.
Journal: J Hypertens | Authors: H Xie | Species: human | Application: WB - Development of Human CBF1-Targeting Single-Stranded DNA Aptamers with Antiangiogenic Activity In Vitro.
Journal: Nucleic Acid Ther | Authors: Mari Tezuka-Kagajo - Three-dimensional live imaging of Atoh1 reveals the dynamics of hair cell induction and organization in the developing cochlea.
Journal: Development | Authors: Tomoko Tateya | Species: mouse | Application: IHC - Endothelial HIF2α suppresses retinal angiogenesis in neonatal mice by upregulating NOTCH signaling
Journal: Development | Authors: Li-Juan Duan | Species: mouse | Application: IF - Id4, a new candidate gene for senile osteoporosis, acts as a molecular switch promoting osteoblast differentiation.
Journal: PLoS Genet | Authors: Tokuzawa Yoshimi Y | Species: mouse | Application: WB - Cadmium Reduces VE-Cadherin Expression in Endothelial Cells Through Activation of the Notch Signaling Pathway
Journal: J Biochem Mol Toxicol | Authors: Yuanqing Zhang | Species: mouse,human | Application: WB - EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway.
Journal: Oxid Med Cell Longev | Authors: Hua Xie | Species: human | Application: WB - Notch3-specific inhibition using siRNA knockdown or GSI sensitizes paclitaxel-resistant ovarian cancer cells.
Journal: Mol Carcinog | Authors: Haeyoun Kang | Species: human | Application: WB - Notch pathway inhibition mediated by arsenic trioxide depletes tumor initiating cells in small cell lung cancer.
Journal: Mol Biol Rep | Authors: Meng-Hang Yang | Species: human | Application: WB - The effect of maternal exposure to di-(2-ethylhexyl)-phthalate on fetal cardiac development in mice.
Journal: J Appl Toxicol | Authors: Changqing Tang | Species: mouse | Application: WB - MTHFD2 drives retinoblastoma progression via Notch signaling activation: Implications for targeted pediatric ocular cancer therapy
Journal: Exp Eye Res | Authors: Chunyue Yao | Application: WB - An important role of endothelial Hairy-related transcription factors in mouse vascular development.
Journal: Genesis | Authors: Takashi Morioka | Species: mouse | Application: IF - Placental P-glycoprotein inhibition enhances susceptibility to Di-(2-ethylhexyl)-phthalate induced cardiac malformations in mice: A possibly promising target for congenital heart defects prevention.
Journal: PLoS One | Authors: Changqing Tang | Species: mouse | Application: WB - Homocysteine causes neuronal leptin resistance and endoplasmic reticulum stress
Journal: PLoS One | Authors: Arini Isnani Preninka | Species: human | Application: WB - GATA6 inhibits the periodontitis progression through regulating the Notch signaling pathway
Journal: Odontology | Authors: Wen-Wen Li - Pharyngeal arch artery defects and lethal malformations of the aortic arch and its branches in mice deficient for the Hrt1/Hey1 transcription factor.
Journal: Mech Dev | Authors: Masahide Fujita | Species: mouse | Application: IHC - Evidence that TD-198946 enhances the chondrogenic potential of human synovium-derived stem cells through the NOTCH3 signaling pathway.
Journal: J Tissue Eng Regen Med | Authors: Masato Kobayashi | Species: human | Application: IF - Loss of myocardial Hey2/Hrt2 function disrupts rightward shift of atrioventricular cushion tissue and causes tricuspid atresia
Journal: Dev Dyn | Authors: Kazuki Okumura | Species: mouse | Application: IHC - Photobiomodulation (800 nm Light-Emitting Diode) Treatment Promotes Bone Mesenchymal Stem Cell Proliferation Via Long Noncoding RNA MEG3-MicroRNA-217-5P Pathway
Journal: Photobiomodul Photomed Laser Surg | Authors: Na Liu | Species: mouse | Application: WB - miR-150 enhances apoptotic and anti-tumor effects of paclitaxel in paclitaxel-resistant ovarian cancer cells by targeting Notch3.
Journal: Oncotarget | Authors: Tae Hoen Kim | Species: human | Application: WB
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