ChromoTek mNeonGreen-Trap Agarose nta proteintech

$289.00
In stock
SKU
nta
Catalog No.SizePrice (USD)
nta-1010rxns$289.00
nta-2020rxns$499.00
nta-100100rxns$2,154.00
nta-200200rxns$3,834.00
nta-400400rxns$6,650.00
SynonymsmNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag
HostAlpaca
FormulationUnited States
ApplicationsIP, CoIP, ChIP, RIP
Host / IsotypeIP, CoIP, ChIP, RIP
Specificity/TargetmNeonGreen For the complete list, please click here: Fluorescent protein specificity table
Binding capacity30 μg of recombinant mNeonGreen per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer 0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
TypeNanobody
ClassRecombinant
Affinity (K D )Dissociation constant K D of 2 nM
Compatibility with mass spectrometryThe mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827593
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!
Size25ul/reactions (eg:20rxns=500ul slurry)
IPPlant J Elucidation of the late steps in the glycan-dependent ERAD of soluble misfolded glycoproteins Authors - Jennifer Schoberer View Article
CoIPDev Cell The ER ladder is a unique morphological feature of developing mammalian axons. Authors - Emiliano Zamponi View Article

Description

Immunoprecipitation of mNeonGreen-fusion proteins and their interacting factors with anti-mNeonGreen Nanobody conjugated to beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Short incubation time of about 60 minutes

Publications

  1. Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit.
    Journal: Cell Stem Cell | Authors: Christopher S Morrow | Species: IP | Application: Cell Stem Cell Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit. Authors - Christopher S Morrow View Article
  2. The ER ladder is a unique morphological feature of developing mammalian axons.
    Journal: Dev Cell | Authors: Emiliano Zamponi | Species: CoIP | Application: Dev Cell The ER ladder is a unique morphological feature of developing mammalian axons. Authors - Emiliano Zamponi View Article
  3. The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export.
    Journal: Proc Natl Acad Sci U S A | Authors: Peiwei Liu | Species: IP | Application: Proc Natl Acad Sci U S A The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export. Authors - Peiwei Liu View Article
  4. The HSPG syndecan is a core organizer of cholinergic synapses.
    Journal: J Cell Biol | Authors: Xin Zhou | Species: IP | Application: J Cell Biol The HSPG syndecan is a core organizer of cholinergic synapses. Authors - Xin Zhou View Article
  5. SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility.
    Journal: J Cell Biol | Authors: Sahezeel Awadia | Species: IP | Application: J Cell Biol SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility. Authors - Sahezeel Awadia View Article
  6. Elucidation of the late steps in the glycan-dependent ERAD of soluble misfolded glycoproteins
    Journal: Plant J | Authors: Jennifer Schoberer | Species: IP | Application: Plant J Elucidation of the late steps in the glycan-dependent ERAD of soluble misfolded glycoproteins Authors - Jennifer Schoberer View Article
  7. The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export.
    Journal: Proc Natl Acad Sci U S A | Authors: Peiwei Liu | Application: IP
  8. The ER ladder is a unique morphological feature of developing mammalian axons.
    Journal: Dev Cell | Authors: Emiliano Zamponi | Application: CoIP
  9. Autophagosomes anchor an AKAP11-dependent regulatory checkpoint that shapes neuronal PKA signaling
    Journal: EMBO J | Authors: Ashley Segura-Roman
  10. The cyclin dependent kinase (CDK)7 inhibitor BS-181 inhibits pathogenic Cryptococcus species, causing G(2)/M arrest and a splicing defect.
    Journal: Virulence | Authors: Pooja Sethiya | Application: IP
  11. Elucidation of the late steps in the glycan-dependent ERAD of soluble misfolded glycoproteins
    Journal: Plant J | Authors: Jennifer Schoberer | Application: IP
  12. The HSPG syndecan is a core organizer of cholinergic synapses.
    Journal: J Cell Biol | Authors: Xin Zhou | Species: C. elegans | Application: IP
  13. SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility.
    Journal: J Cell Biol | Authors: Sahezeel Awadia | Application: IP
  14. Functional insight into cyclin-dependent kinase (CDK)7 via chemical inhibition of the priority fungal pathogen Cryptococcus neoformans.
    Journal: mBio | Authors: Pooja Sethiya | Application: IP
  15. Kaposi's sarcoma-associated herpesvirus ORF61 protein sequesters APOBEC3B in filamentous aggregates
    Journal: J Virol | Authors: Laura-Marie Luoto | Application: IP
  16. BubR1 recruitment to the kinetochore via Bub1 enhances spindle assembly checkpoint signaling
    Journal: Mol Biol Cell | Authors: Anand Banerjee | Application: IP
  17. Cvm1 and its paralog Cvm2 function as a complex at vacuolar membrane contact sites
    Journal: Mol Biol Cell | Authors: Daniel D Bisinski
  18. Phosphorylation Protects Oncogenic RAS from LZTR1-Mediated Degradation.
    Journal: bioRxiv | Authors: Lin Zhang | Application: CoIP
  19. Autophagosomes coordinate an AKAP11-dependent regulatory checkpoint that shapes neuronal PKA signaling
    Journal: bioRxiv | Authors: Ashley Segura-Roman | Application: IP
  20. TREX2 component PCID2 scaffolds alternative SAC3-based subcomplexes with distinct RNA processing and export function.
    Journal: bioRxiv | Authors: Vasilisa Aksenova | Application: IP
  21. Phosphorylation Protects Oncogenic RAS from LZTR1-Mediated Degradation.
    Journal: bioRxiv | Authors: Lin Zhang | Application: CoIP

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