| Catalog Number | 10745-1-AP |
| Synonyms | NF κB, p65, p65; RELA, RELA, NF Kappa B |
| Host | Rabbit |
| Reactivity | human and More (8) |
| Reactivity | human, canine, chicken, bovine, hamster, goat, fish, bombyx mori, duck |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human breast cancer tissue, human colon tissue, human liver cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells, TNF alpha treated HT-1080 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human colon tissue, human liver cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, TNF alpha treated HT-1080 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human |
| Cited Reactivity | human, canine, chicken, bovine, hamster, goat, fish, bombyx mori, duck |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag1199 Product name: Recombinant human p65; RELA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-220 aa of BC011603 Sequence: MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQ Predict reactive species |
| Full Name | v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
| Calculated Molecular Weight | 65 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC011603 |
| Gene Symbol | NF-κB p65 |
| Gene ID (NCBI) | 5970 |
| RRID | AB_2178878 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q04206 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
| IHC protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
| IP protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
| WB protocol for NF-κB p65 antibody 10745-1-AP | Download protocol |
| human | WB,IF J Hematol Oncol Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFκB-TLR signaling pathway. Authors - Xiaoxia Li View Article |
| Karthick (Verified Customer) (04-08-2026) | 1 in 1000 dilution, NFKB antibody works well Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Cell culture Primary Antibody Dilution: 1:1000 dilution Cell Tissue Type: Astrocytes and Microglia cells NF-κB p65 Antibody Western Blot,Immunohistochemistry,Immunofluorescence,Cell culture validation (1:1000 dilution dilution) in Astrocytes and Microglia cells (Cat no:10745-1-AP) |
| Mounika (Verified Customer) (03-24-2026) | Very nice antibody, good to work with it1 Applications: Western Blot, Immunohistochemistry, Immunofluorescence Primary Antibody Dilution: 1:1000 |
| Vignesh (Verified Customer) (11-18-2025) | Applications: Western Blot Primary Antibody Dilution: 1:500 Cell Tissue Type: THP-1 CELLS |
| Maximilian (Verified Customer) (06-30-2023) | Quite a bit of unspecific binding. Did not work in mouse cell lines. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Mainly Human Breast Cancer Cell lines (MDA-MB-436, MDA-MB-468, MDA-MB-231) |
| Xie (Verified Customer) (09-29-2022) | NF-κB p65 Antibody staining. Applications: Immunofluorescence Primary Antibody Dilution: 1:250 Cell Tissue Type: hela NF-κB p65 Antibody Immunofluorescence validation (1:250 dilution) in hela (Cat no:10745-1-AP) |
| Murali (Verified Customer) (01-24-2022) | Very nice antibody for IHC Applications: Immunohistochemistry Primary Antibody Dilution: 1 to 200 Cell Tissue Type: pharyngeal mucosal NF-κB p65 Antibody Immunohistochemistry validation (1 to 200 dilution) in pharyngeal mucosal (Cat no:10745-1-AP) |
| Azita (Verified Customer) (05-31-2021) | NSC-34 cells (motor neuron-like cells) strong labelling in WB Applications: Western Blot Primary Antibody Dilution: 1/2000 Cell Tissue Type: Human spinal cord (cervical) tissue |
| Zehua (Verified Customer) (12-17-2019) | After siRNA treatment for 48 hours, specific signal shown here. Works excellent! Applications: Western Blot, Cell culture, Primary Antibody Dilution: 1:1000 Cell Tissue Type: K562 NF-κB p65 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in K562 (Cat no:10745-1-AP) |
| Feng-qian (Verified Customer) (12-07-2018) | Applications: Western Blot, Immunofluorescence, Primary Antibody Dilution: 1/500 Cell Tissue Type: HaCaT |
| Fraser (Verified Customer) (06-06-2018) | Applications: Other (please state below), |
| Citations | 2387 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA. Protocols Product Specific Protocols IF protocol for NF-κB p65 antibody 10745-1-AP Download protocol IHC protocol for NF-κB p65 antibody 10745-1-AP Download protocol IP protocol for NF-κB p65 antibody 10745-1-AP Download protocol WB protocol for NF-κB p65 antibody 10745-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
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