CoraLite® Plus 488-conjugated pan-keratin Polyclonal antibody CL488-26411 proteintech
$479.00
In stock
SKU
CL488-26411
| Catalog Number | CL488-26411 |
| Synonyms | Pan CK, Pan Keratin, pan-CK, CK 5, CK-5 |
| Host | Rabbit |
| Reactivity | human |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | IF/ICC, IF-P, FC (Intra) |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF-P detected in | human colon cancer tissue, human lung cancer tissue, human rectal cancer tissue |
| Tested Applications — Positive IF/ICC detected in | A431 cells, HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | A431 cells |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive IF-P detected in | human colon cancer tissue, human lung cancer tissue, human rectal cancer tissue |
| Positive IF/ICC detected in | A431 cells, HeLa cells |
| Positive FC (Intra) detected in | A431 cells |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag24184 Product name: Recombinant human KRT5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 316-491 aa of BC024292 Sequence: THVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNI Predict reactive species |
| Full Name | keratin 5 |
| Calculated Molecular Weight | 590 aa, 62 kDa |
| GenBank Accession Number | BC024292 |
| Gene Symbol | Cytokeratin 5 |
| Gene ID (NCBI) | 3852 |
| RRID | AB_2919208 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13647 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL Plus 488 pan-keratin antibody CL488-26411 | Download protocol |
| IF protocol for CL Plus 488 pan-keratin antibody CL488-26411 | Download protocol |
| Citations | - |
| Dilutions | IF-P : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | CoraLite® Plus 488 |
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody. Protocols Product Specific Protocols FC protocol for CL Plus 488 pan-keratin antibody CL488-26411 Download protocol IF protocol for CL Plus 488 pan-keratin antibody CL488-26411 Download protocol Standard Protocols Click here to view our Standard Protocols
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