CoraLite®594-conjugated Alpha smooth muscle actin specific Recombinant monoclonal antibody CL594-80008 proteintech
$479.00
In stock
SKU
CL594-80008
| Catalog Number | CL594-80008 |
| Synonyms | ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 5H7 |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig, chicken |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | WB, IF/ICC, IF-P, IF-Fro, FC (Intra) |
| Applications | IF/ICC, IF-P, IF-Fro, FC (Intra) |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive IF-P detected in | mouse heart tissue |
| Tested Applications — Positive IF-Fro detected in | mouse small intestine tissue |
| Tested Applications — Positive IF/ICC detected in | H9C2 cells |
| Tested Applications — Positive FC (Intra) detected in | C2C12 cells |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF-Fro detected in | mouse small intestine tissue |
| Positive IF/ICC detected in | H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Tested Reactivity | human, mouse, rat, pig, chicken |
| Cited Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_2934798 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Purification Method | Protein A purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| FC protocol for CL594 Alpha smooth muscle actin specific antibody CL594-80008 | Download protocol |
| IF protocol for CL594 Alpha smooth muscle actin specific antibody CL594-80008 | Download protocol |
| human | WB Toxicol Appl Pharmacol Mechanistic role for mTORC1 signaling in profibrotic toxicity of low-dose cadmium Authors - Choon-Myung Lee View Article |
| human,mouse | WB Chem Biodivers UPLC-MS/MS-Network Pharmacology and Experimental Validation Reveal Active Components of Ficus tikoua for Ameliorating Liver Fibrosis. Authors - Shi-Ying Xu View Article |
| Citations | 2 |
| Dilutions | IF-P : 1:50-1:500 IF-FRO : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 5H7 |
| Conjugation | CoraLite®594 |
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. Protocols Product Specific Protocols FC protocol for CL594 Alpha smooth muscle actin specific antibody CL594-80008 Download protocol IF protocol for CL594 Alpha smooth muscle actin specific antibody CL594-80008 Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Mechanistic role for mTORC1 signaling in profibrotic toxicity of low-dose cadmium
Journal: Toxicol Appl Pharmacol | Authors: Choon-Myung Lee | Species: human | Application: WB - UPLC-MS/MS-Network Pharmacology and Experimental Validation Reveal Active Components of Ficus tikoua for Ameliorating Liver Fibrosis.
Journal: Chem Biodivers | Authors: Shi-Ying Xu | Species: human,mouse | Application: WB
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
