ASAP3 Polyclonal antibody 14099-1-AP proteintech
$149.00
In stock
SKU
14099-1-AP
| Catalog Number | 14099-1-AP |
| Synonyms | Development and differentiation-enhancing factor-like 1, DDEFL1, CENTB6, ASAP 3, Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3 |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, L02 cells |
| Tested Applications — Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | HepG2 cells, L02 cells |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag5231 Product name: Recombinant human ASAP3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 558-903 aa of BC060786 Sequence: RDLLSVLEAFANGQDFGQPLPGPDAQAPEELVLHLAVKVANQASLPLVDFIIQNGGHLDAKAADGNTALHYAALYNQPDCLKLLLKGRALVGTVNEAGETALDIARKKHHKECEELLEQAQAGTFAFPLHVDYSWVISTEPGSDSEEDEEEKRCLLKLPAQAHWASGRLDISNKTYETVASLGAATPQGESEDCPPPLPVKNSSRTLVQGCARHASGDRSEVSSLSSEAPETPESLGSPASSSSLMSPLEPGDPSQAPPNSEEGLREPPGTSRPSLTSGTTPSEMYLPVRFSSESTRSYRRGARSPEDGPSARQPLPRRNVPVGITEGDGSRTGSLPASSVQLLQD Predict reactive species |
| Full Name | ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 |
| Calculated Molecular Weight | 903 aa, 99 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC060786 |
| Gene Symbol | ASAP3 |
| Gene ID (NCBI) | 55616 |
| RRID | AB_2059021 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TDY4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ASAP3 antibody 14099-1-AP | Download protocol |
| IHC protocol for ASAP3 antibody 14099-1-AP | Download protocol |
| WB protocol for ASAP3 antibody 14099-1-AP | Download protocol |
| human | WB, IHC Aging (Albany NY) Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes. Authors - Jiaming Fan View Article |
| Mouse | WB,IHC J Cell Mol Med Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes. Authors - Hao Wang View Article |
| Citations | 2 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
ArfGAP with SH3 domain, ankyrin repeat, and PH domain 3 (ASAP3) is a specific ARF6 GTPase-activating protein (GAP), also termed DDEFL1 and ACAP4, which comprises ArfGAP and ankyrin repeat (ANK) domains in the C-terminus and BAR and PH domains in the N-terminus.ASAP3 is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling. Protocols Product Specific Protocols IF protocol for ASAP3 antibody 14099-1-AP Download protocol IHC protocol for ASAP3 antibody 14099-1-AP Download protocol WB protocol for ASAP3 antibody 14099-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.
Journal: Aging (Albany NY) | Authors: Jiaming Fan | Species: human | Application: WB, IHC - Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes.
Journal: J Cell Mol Med | Authors: Hao Wang | Species: Mouse | Application: WB,IHC
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