ATP1A1/2 Monoclonal antibody 68505-1-Ig proteintech
$149.00
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68505-1-Ig
| Catalog Number | 68505-1-Ig |
| Synonyms | ATP1A2, 3D8G1, alpha 2 ATPase, ATP1A 2, EC:7.2.2.13 |
| Host | Mouse |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, IP, ELISA |
| Applications | WB, IF/ICC, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | rat brain tissue, SK-BR-3 cells, rat kidney tissue, pig brain tissue, mouse brain tissue |
| Tested Applications — Positive IF/ICC detected in | C2C12 cells, HEK-293 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Positive WB detected in | rat brain tissue, SK-BR-3 cells, rat kidney tissue, pig brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | C2C12 cells, HEK-293 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Tested Reactivity | human, mouse, rat, pig |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag10489 Product name: Recombinant human ATP1A2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 347-700 aa of BC052271 Sequence: KRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGATFDKRSPTWTALSRIAGLCNRAVFKAGQENISVSKRDTAGDASESALLKCIELSCGSVRKMRDRNPKVAEIPFNSTNKYQLSIHEREDSPQSHVLVMKGAPERILDRCSTILVQGKEIPLDKEMQDAFQNAYMELGGLGERVLGFCQLNLPSGKFPRGFKFDTDELNFPTEKLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPMSQVNPREAKACVVHGSDLKDMTSEQLDEILKNHTEIVFARTSPQQKLIIVE Predict reactive species |
| Full Name | ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| Calculated Molecular Weight | 1020 aa, 112 kDa |
| Observed Molecular Weight | 97-100 kDa |
| GenBank Accession Number | BC052271 |
| Gene Symbol | ATP1A2 |
| Gene ID (NCBI) | 477 |
| RRID | AB_3085215 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | P50993 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ATP1A1/2 antibody 68505-1-Ig | Download protocol |
| WB protocol for ATP1A1/2 antibody 68505-1-Ig | Download protocol |
| Citations | 1 |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:500-1:2000 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 3D8G1 |
| Conjugation | Unconjugated |
ATP1A1/2 is the catalytic component of the active enzyme Na+/K+-ATPase, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. The Na+/K+-ATPase is composed of a larger catalytic α-subunit (~110 kDa) and a small β-subunit (~55 kDa). The α subunit has four isoforms identified to date: α1, α2, α3 and α4.The α1 isoform is expressed ubiquitously but the α2 isoform is present largely in the skeletal muscle, heart and vascular smooth muscle. The α3 isoform is found almost exclusively in neurons and ovaries. The α4 isoform is expressed in sperm. This antibody can recognize ATP1A1 and ATP1A2. Protocols Product Specific Protocols IF protocol for ATP1A1/2 antibody 68505-1-Ig Download protocol WB protocol for ATP1A1/2 antibody 68505-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- AAVLINK: A potent DNA-recombination method for large cargo delivery in gene therapy.
Journal: Cell | Authors: Jianbang Lin
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