TGN46 Polyclonal antibody 13573-1-AP proteintech
$149.00
In stock
SKU
13573-1-AP
| Catalog Number | 13573-1-AP |
| Synonyms | TGOLN2, hTGN46, hTGN48, hTGN51, TGN38 |
| Host | Rabbit |
| Reactivity | human and More (2) |
| Reactivity | human, monkey, hamster |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | A549 cells, HepG2 cells, Calu-1 cells, LNCaP cells |
| Tested Applications — Positive IP detected in | HeLa cells |
| Tested Applications — Positive IHC detected in | human stomach cancer, human liver tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | human cerebellum tissue |
| Tested Applications — Positive IF/ICC detected in | HeLa cells, A549 cells, U2OS cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Positive WB detected in | A549 cells, HepG2 cells, Calu-1 cells, LNCaP cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach cancer, human liver tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human cerebellum tissue |
| Positive IF/ICC detected in | HeLa cells, A549 cells, U2OS cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| Tested Reactivity | human |
| Cited Reactivity | human, monkey, hamster |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag4470 Product name: Recombinant human TGOLN2,TGN46 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 25-384 aa of BC028219 Sequence: SVKQEEAGVRPSAGNVSTHPSLSQRPGGSTKSHPEPQTPKDSPSKSSAEAQTPEDTPNKSGAEAKTQKDSSNKSGAEAKTQKGSTSKSGSEAQTTKDSTSKSHPELQTPKDSTGKSGAEAQTPEDSPNRSGAEAKTQKDSPSKSGSEAQTTKDVPNKSGADGQTPKDGSSKSGAEDQTPKDVPNKSGAEKQTPKDGSNKSGAEEQGPIDGPSKSGAEEQTSKDSPNKVVPEQPSRKDHSKPISNPSDNKELPKADTNQLADKGKLSPHAFKTESGEETDLISPPQEEVKSSEPTEDVEPKEAEDDDTGPEEGSPPKEEKEKMSGSASSENREGTLSDSTGSEKDDLYPNGSGNGSAESSH Predict reactive species |
| Full Name | trans-golgi network protein 2 |
| Calculated Molecular Weight | 447 aa, 47 kDa |
| Observed Molecular Weight | 90-100 kDa |
| GenBank Accession Number | BC028219 |
| Gene Symbol | TGN46 |
| Gene ID (NCBI) | 10618 |
| RRID | AB_10597396 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43493 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for TGN46 antibody 13573-1-AP | Download protocol |
| IHC protocol for TGN46 antibody 13573-1-AP | Download protocol |
| IP protocol for TGN46 antibody 13573-1-AP | Download protocol |
| WB protocol for TGN46 antibody 13573-1-AP | Download protocol |
| human | WB Proc Natl Acad Sci U S A Monensin suppresses EMT-driven cancer cell motility by inducing Golgi pH-dependent exocytosis of GOLIM4 Authors - Xiaochao Tan KD Validated View Article |
| Sammy (Verified Customer) (01-20-2024) | A good antibody for IF and also works for WB. Applications: Western Blot, Immunofluorescence Primary Antibody Dilution: 1 in 1000 Cell Tissue Type: HUVEC |
| Amy (Verified Customer) (08-10-2023) | Nice Golgi staining of HEK293T cells. Applications: Immunofluorescence Primary Antibody Dilution: 1:100 Cell Tissue Type: HEK293T |
| Christine (Verified Customer) (01-27-2023) | Detects 2 bands around the 85 kDa marker, a sharp one just below and a more fuzzy one just above. Applications: Western Blot Primary Antibody Dilution: 1:2,500 Cell Tissue Type: HEK293T |
| Thomas (Verified Customer) (09-18-2020) | HEK293T cells were fixed in 4% PFA for 15 mins and permeabilised in 0.1% Triton-X 100 in PBS. Cells were blocked in 1% BSA. Primary antibody solution was diluted at 1:200 in blocking solution and incubated for 1 hour. Goat anti-rabbit 647 Alexa Fluor secondary antibody (1:250 - red) and DAPI (1:2000 - blue) were incubated with cells for 1 hour. Applications: Immunofluorescence Primary Antibody Dilution: 1:200 TGN46 Antibody Immunofluorescence validation (1:200 dilution) in (Cat no:13573-1-AP) |
| Stephen (Verified Customer) (10-01-2019) | RPE cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours TGN46 antibody(green) incubated 1:300 and EEA1 antibody (purple) overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) visualize DNA Applications: Immunofluorescence, Primary Antibody Dilution: 1:300 Cell Tissue Type: RPE (human) TGN46 Antibody Immunofluorescence, validation (1:300 dilution) in RPE (human) (Cat no:13573-1-AP) |
| Citations | 58 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 IF-P : 1:50-1:500 IF/ICC : 1:1000-1:4000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TGN46 (TGOLN2), the human homolog of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively, spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations. Protocols Product Specific Protocols IF protocol for TGN46 antibody 13573-1-AP Download protocol IHC protocol for TGN46 antibody 13573-1-AP Download protocol IP protocol for TGN46 antibody 13573-1-AP Download protocol WB protocol for TGN46 antibody 13573-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Biowire Model of Interstitial and Focal Cardiac Fibrosis.
Journal: ACS Cent Sci | Authors: Erika Yan Wang - Aberrant phase separation drives membranous organelle remodeling and tumorigenesis
Journal: Mol Cell | Authors: Xinyu Wang - Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor.
Journal: Autophagy | Authors: Amy H Ponsford | Species: human | Application: IF - Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade.
Journal: Cell Death Differ | Authors: Giulia Cerrato | Species: human | Application: IF - Bunyavirus SFTSV exploits autophagic flux for viral assembly and egress
Journal: Autophagy | Authors: Jia-min Yan | Species: human | Application: WB - Monensin suppresses EMT-driven cancer cell motility by inducing Golgi pH-dependent exocytosis of GOLIM4
Journal: Proc Natl Acad Sci U S A | Authors: Xiaochao Tan KD Validated | Species: human | Application: WB - Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane
Journal: Nat Commun | Authors: Tong Chen | Species: human | Application: IF - Regulation of cellular cholesterol distribution via non-vesicular lipid transport at ER-Golgi contact sites
Journal: Nat Commun | Authors: Tomoki Naito | Species: human | Application: IF - CRISPR screens and lectin microarrays identify high mannose N-glycan regulators
Journal: Nat Commun | Authors: C Kimberly Tsui | Species: human | Application: IF - Aberrant phase separation drives membranous organelle remodeling and tumorigenesis
Journal: Mol Cell | Authors: Xinyu Wang - YTHDF1 differentiates the contributing roles of mTORC1 in aging
Journal: Mol Cell | Authors: Chenzhong Xu | Species: human | Application: WB - Dual-Organelle Secretome Conjugation and Organ-Uptake Tracking (DuO-SCOUT) Enables Deep and Sensitive Mapping of the Secreted Proteins.
Journal: Adv Sci (Weinh) | Authors: Fenglian Yang - The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection.
Journal: Sci Adv | Authors: Tai-Ting Woo | Species: human | Application: WB - ITCH regulates Golgi integrity and proteotoxicity in neurodegeneration.
Journal: Sci Adv | Authors: Qiwang Xiang | Species: human | Application: WB,IF - Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade.
Journal: Cell Death Differ | Authors: Giulia Cerrato | Species: human | Application: IF - Synaptic and extrasynaptic distribution of NMDA receptors in the cortex of Alzheimer's disease patients
Journal: Alzheimers Dement | Authors: Sergio Escamilla | Application: WB - Deltex E3 ubiquitin ligase 2 potentiates STING-mediated type I interferon response by K63-linked ubiquitination.
Journal: Cell Death Dis | Authors: Zhuang Liu | Application: IF - Biowire Model of Interstitial and Focal Cardiac Fibrosis.
Journal: ACS Cent Sci | Authors: Erika Yan Wang - A new tau dephosphorylation-targeting chimera for the treatment of tauopathies
Journal: Acta Pharmacol Sin | Authors: Jing-Fen Su | Application: IF - DMXL1 promotes recruitment of V1-ATPase to lysosomes upon TRPML1 activation
Journal: Nat Struct Mol Biol | Authors: Chan Lee | Species: human | Application: IF - Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging
Journal: Nat Struct Mol Biol | Authors: Rui Guo | Species: human | Application: IF - Monensin suppresses EMT-driven cancer cell motility by inducing Golgi pH-dependent exocytosis of GOLIM4
Journal: Proc Natl Acad Sci U S A | Authors: Xiaochao Tan | Species: human | Application: WB - TM9SF3 is a Golgi-resident ATG8-binding protein essential for Golgi-selective autophagy
Journal: Dev Cell | Authors: Jiejie Yang - PIKfyve inhibition induces antitumor immunogenicity by attenuating STING trafficking and lysosomal degradation
Journal: Cancer Immunol Res | Authors: Jie He | Species: human | Application: WB,IF - An Efficient Bivalent Cyclic RGD-PIK3CB siRNA Conjugate for Specific Targeted Therapy against Glioblastoma In Vitro and In Vivo.
Journal: Mol Ther Nucleic Acids | Authors: Bohong Cen | Species: human | Application: IF - The Salmonella Typhimurium effector SpvB subverts host membrane trafficking by targeting clathrin and AP-1
Journal: Mol Cell Proteomics | Authors: Yi Yuan | Species: human | Application: IF - Rab32 family proteins regulate autophagosomal components recycling
Journal: J Cell Biol | Authors: Zhe Wu | Species: human | Application: WB - ULK phosphorylation of STX17 controls autophagosome maturation via FLNA
Journal: J Cell Biol | Authors: Yufen Wang | Species: human | Application: WB - VPS13B is localized at the interface between Golgi cisternae and is a functional partner of FAM177A1
Journal: J Cell Biol | Authors: Berrak Ugur | Species: monkey | Application: IF - GP73-mediated secretion of AFP and GP73 promotes proliferation and metastasis of hepatocellular carcinoma cells.
Journal: Oncogenesis | Authors: Yujuan Liu | Species: human | Application: WB - A conserved ion channel function of STING mediates noncanonical autophagy and cell death
Journal: EMBO Rep | Authors: Jinrui Xun | Species: human | Application: IF - GP73-mediated secretion of AFP and GP73 promotes proliferation and metastasis of hepatocellular carcinoma cells.
Journal: Oncogenesis | Authors: Yujuan Liu | Species: human | Application: WB - COPB1-knockdown induced type I interferon signaling activation inhibits Chlamydia psittaci intracellular proliferation
Journal: Front Microbiol | Authors: Nana Li | Species: human | Application: WB - Cycloheptylprodigiosin from marine bacterium Spartinivicinus ruber MCCC 1K03745T induces a novel form of cell death characterized by Golgi disruption and enhanced secretion of cathepsin D in Non-small cell lung cancer cell lines
Journal: Eur J Pharmacol | Authors: Xiaosi Lin | Species: human | Application: WB,IF - Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.
Journal: Int J Mol Sci | Authors: Bożena Szulc | Species: human | Application: IF - Presenilin 1 Modulates Acetylcholinesterase Trafficking and Maturation
Journal: Int J Mol Sci | Authors: María-Ángeles Cortés-Gómez | Species: hamster | Application: IF - Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter.
Journal: Int J Mol Sci | Authors: Bożena Szulc | Species: human | Application: IF - Intracellular Group A Streptococcus Induces Golgi Fragmentation To Impair Host Defenses through Streptolysin O and NAD-Glycohydrolase.
Journal: mBio | Authors: Takashi Nozawa | Species: human | Application: IF - Rab6a enables BICD2/dynein-mediated trafficking of human papillomavirus from the trans-Golgi network during virus entry
Journal: mBio | Authors: Jeongjoon Choi | Species: human | Application: IF - Host RAB11FIP5 protein inhibits the release of Kaposi's sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45.
Journal: PLoS Pathog | Authors: Xiaoqin Wei | Species: human | Application: IF - Cryo-EM structure of the WDR11-FAM91A1-C17orf75 complex involved in retrograde trafficking.
Journal: Structure | Authors: Yifan Zhang - Synaptic Vesicle Glycoprotein 2A Slows down Amyloidogenic Processing of Amyloid Precursor Protein via Regulating Its Intracellular Trafficking
Journal: Biomed Environ Sci | Authors: Qian Zhang | Species: human | Application: WB,IF - Subcellular dynamics and functional activity of the cleaved intracellular domain of the Na+ channel β1 subunit.
Journal: J Biol Chem | Authors: Alexander S Haworth | Species: human | Application: IF - N-terminal acetyltransferase 6 facilitates enterovirus 71 replication by regulating PI4KB expression and replication organelle biogenesis
Journal: J Virol | Authors: Hang Yang | Species: human | Application: WB - Lactate-lactylation-HSPA6 axis promotes PRRSV replication by impairing IFN-β production
Journal: J Virol | Authors: Yu Pang | Application: IF - SARS-CoV-2 protein ORF8 limits expression levels of Spike antigen and facilitates immune evasion of infected host cells
Journal: J Biol Chem | Authors: Ik-Jung Kim | Species: human | Application: IF - S-palmitoylation determines TMEM55B-dependent positioning of lysosomes
Journal: J Cell Sci | Authors: Sönke Rudnik - Cell death induced by nicotine in human neuroblastoma SH-SY5Y cells is mainly attributed to cytoplasmic vacuolation originating from the trans-Golgi network
Journal: Food Chem Toxicol | Authors: Jun Li | Species: human | Application: IF - Novel determinants of NOTCH1 trafficking and signaling in breast epithelial cells
Journal: Life Sci Alliance | Authors: Francis M Kobia | Species: Human | Application: IF - Visualized procollagen Iα1 demonstrates the intracellular processing of propeptides.
Journal: Life Sci Alliance | Authors: Toshiaki Tanaka | Application: IF - Identification of novel potential interaction partners of UDP-galactose (SLC35A2), UDP-N-acetylglucosamine (SLC35A3) and an orphan (SLC35A4) nucleotide sugar transporters.
Journal: J Proteomics | Authors: Maciej Wiktor | Species: human | Application: IF - Taming Bacterial Toxins into an Anticancer Platform.
Journal: Biochem Biophys Res Commun | Authors: Chen Yang | Species: human | Application: IF - A Novel Missense Variant in Ultrarare SLC35A1-CDG Alters Cellular Glycosylation, Lipid, and Energy Metabolism Without Affecting CDG Serum Markers.
Journal: Hum Mutat | Authors: Kristina Falkenstein | Species: human | Application: WB - Protocol for immunostaining of non-adherent cells and cellular structures using centrifugal filter devices
Journal: STAR Protoc | Authors: Momoko Miyazawa | Species: human | Application: IF - Combinatorial CRISPR screens and lectin microarrays identify novel glycosylation regulators
Journal: bioRxiv | Authors: C Kimberly Tsui | Species: human | Application: IF - Targeted Protein Unfolding at the Golgi Apparatus
Journal: Methods Mol Biol | Authors: Jasmin Schillinger - Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists.
Journal: bioRxiv | Authors: L. Robert Hollingsworth | Species: human | Application: IF - Immunofluorescence Microscopy of the Mammalian Golgi Apparatus
Journal: Methods Mol Biol | Authors: Maryam Arab | Species: human | Application: IF - Cell-cycle-resolved τSTED nanoscopy reveals nanoscale reorganization of TGN46-positive structures.
Journal: Cell Struct Funct | Authors: Kaoru Katoh | Species: human | Application: IF
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