ATPAF1 Polyclonal antibody 18016-1-AP proteintech
$149.00
In stock
SKU
18016-1-AP
| Catalog Number | 18016-1-AP |
| Synonyms | ATP11, ATP11 homolog, ATP11p |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HEK-293 cells, PC-3 cells, mouse heart tissue, mouse kidney tissue, rat heart tissue, |
| Tested Applications — Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | HEK-293 cells, PC-3 cells, mouse heart tissue, mouse kidney tissue, rat heart tissue, |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:500-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag12557 Product name: Recombinant human ATPAF1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 25-328 aa of BC071731 Sequence: GLCAVRSRALGLGLVSPAQLRVFPVRPGSGRPEGGADGSGVGAEAELQANPFYDRYRDKIQLLRRSDPAAFESRLEKRSEFRKQPVGHSRQGDFIKCVEQKTDALGKQSVNRGFTKDKTLSSIFNIEMVKEKTAEEIKQIWQQYFAAKDTVYAVIPAEKFDLIWNRAQSCPTFLCALPRREGYEFFVGQWTGTELHFTALINIQTRGEAAASQLILYHYPELKEEKGIVLMTAEMDSTFLNVAEAQCIANQVQLFYATDRKETYGLVETFNLRPNEFKYMSVIAELEQSGLGAELKCAQNQNKT Predict reactive species |
| Full Name | ATP synthase mitochondrial F1 complex assembly factor 1 |
| Calculated Molecular Weight | 328 aa, 36 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC071731 |
| Gene Symbol | ATPAF1 |
| Gene ID (NCBI) | 64756 |
| RRID | AB_2243352 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TC12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ATPAF1 antibody 18016-1-AP | Download protocol |
| IHC protocol for ATPAF1 antibody 18016-1-AP | Download protocol |
| WB protocol for ATPAF1 antibody 18016-1-AP | Download protocol |
| human | WB iScience Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis Authors - Yunfan He View Article |
| mouse | WB Am J Physiol Endocrinol Metab Exogenous H2S reduces acetylation levels of mitochondrial respiratory enzymes via regulating NAD+-SIRT3 pathway in cardiac tissues of db/db mice. Authors - Yu Sun View Article |
| Citations | 6 |
| Dilutions | WB : 1:500-1:4000 IHC : 1:50-1:500 IF-P : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- CA9 Silencing Promotes Mitochondrial Biogenesis, Increases Putrescine Toxicity and Decreases Cell Motility to Suppress ccRCC Progression.
Journal: Int J Mol Sci | Authors: Jiatong Xu | Species: human | Application: WB - Exogenous H2S reduces acetylation levels of mitochondrial respiratory enzymes via regulating NAD+-SIRT3 pathway in cardiac tissues of db/db mice.
Journal: Am J Physiol Endocrinol Metab | Authors: Yu Sun | Species: mouse | Application: WB - The ALDH2/PolG2 axis enhances mitochondrial biogenesis via transcriptional regulation of Nrf2 and promotes chemotherapy resistance in acute myeloid leukaemia.
Journal: Cell Death Dis | Authors: Xiuying Hu | Species: human | Application: WB - Assembly of the catalytic module and the rotor of human ATP synthase.
Journal: EMBO J | Authors: Jiaya He - FAM3A maintains metabolic homeostasis by interacting with F1-ATP synthase to regulate the activity and assembly of ATP synthase
Journal: Metabolism | Authors: Han Yan | Species: human | Application: WB - Deficient tRNA posttranscription modification dysregulated the mitochondrial quality controls and apoptosis
Journal: iScience | Authors: Yunfan He | Species: human | Application: WB
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