ATPBD3 Recombinant monoclonal antibody 84318-4-RR proteintech
$149.00
In stock
SKU
84318-4-RR
| Catalog Number | 84318-4-RR |
| Synonyms | 241630C4, CTU1, NCS6 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | PC-3 cells, K-562 cells, HeLa cells, HEK-293 cells |
| Tested Applications — Positive IHC detected in | mouse kidney tissue, rat brain tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse kidney tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:10000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | PC-3 cells, K-562 cells, HeLa cells, HEK-293 cells |
| Positive IHC detected in | mouse kidney tissue, rat brain tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag35037 Product name: Recombinant human ATPBD3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 50-200 aa of NM_145232 Sequence: PGAVVAVGASGGKDSTVLAHVLRALAPRLGISLQLVAVDEGIGGYRDAALAAVRRQAARWELPLTVVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARRVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGGLGS Predict reactive species |
| Full Name | ATP binding domain 3 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_145232 |
| Gene Symbol | ATPBD3 |
| Gene ID (NCBI) | 90353 |
| RRID | AB_3671859 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q7Z7A3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for ATPBD3 antibody 84318-4-RR | Download protocol |
| IHC protocol for ATPBD3 antibody 84318-4-RR | Download protocol |
| WB protocol for ATPBD3 antibody 84318-4-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:2000-1:10000 IHC : 1:50-1:500 IF-P : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241630C4 |
| Conjugation | Unconjugated |
ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation. Protocols Product Specific Protocols IF protocol for ATPBD3 antibody 84318-4-RR Download protocol IHC protocol for ATPBD3 antibody 84318-4-RR Download protocol WB protocol for ATPBD3 antibody 84318-4-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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