AUH Recombinant monoclonal antibody 85421-1-RR proteintech
$149.00
In stock
SKU
85421-1-RR
| Catalog Number | 85421-1-RR |
| Synonyms | 242854F12, 3-MG-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, EC:4.2.1.18 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF-P, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue, fetal human brain tissue, human kidney tissue |
| Tested Applications — Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF-P detected in | mouse brain tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Recommended Dilutions — Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Positive WB detected in | HeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue, fetal human brain tissue, human kidney tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag11265 Product name: Recombinant human AUH protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-310 aa of BC020722 Sequence: MAAAVAAAPGALGSLHAGGARLVAACSAWLCPGLRLPGSLAGRRAGPAIWAQGWVPAAGGPAPKRGYSSEMKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRYKGE Predict reactive species |
| Full Name | AU RNA binding protein/enoyl-Coenzyme A hydratase |
| Calculated Molecular Weight | 310 aa, 33 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC020722 |
| Gene Symbol | AUH |
| Gene ID (NCBI) | 549 |
| RRID | AB_3744204 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13825 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for AUH antibody 85421-1-RR | Download protocol |
| IHC protocol for AUH antibody 85421-1-RR | Download protocol |
| WB protocol for AUH antibody 85421-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IHC : 1:400-1:1600 IF-P : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 242854F12 |
| Conjugation | Unconjugated |
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