Acetyl-Histone H3 (Lys4) Recombinant monoclonal antibody 84831-1-RR proteintech
$149.00
In stock
SKU
84831-1-RR
| Catalog Number | 84831-1-RR |
| Synonyms | Ac Histone H3, Ac Histone H3 (Lys4), Ac Histone H3 Lys4, Ac Histone H3-Lys4, Acetyl Histone |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, FC (Intra), Dot Blot, ELISA, ChIP-qPCR |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | Trichostatin A treated NIH/3T3 cells, Trichostatin A treated HeLa cells |
| Tested Applications — Positive IF/ICC detected in | Trichostatin A treated HeLa cells, HeLa cells |
| Tested Applications — Positive FC (Intra) detected in | Trichostatin A treated NIH/3T3 cells |
| Tested Applications — Positive Dot Blot detected in | peptide |
| Tested Applications — Positive ChIP-qPCR detected in | HeLa cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Recommended Dilutions — Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| Positive WB detected in | Trichostatin A treated NIH/3T3 cells, Trichostatin A treated HeLa cells |
| Positive IF/ICC detected in | Trichostatin A treated HeLa cells, HeLa cells |
| Positive FC (Intra) detected in | Trichostatin A treated NIH/3T3 cells |
| Positive Dot Blot detected in | peptide |
| Positive ChIP-qPCR detected in | HeLa cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| Tested Reactivity | human, mouse |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | histone cluster 1, H3a |
| Observed Molecular Weight | 15 kDa |
| GenBank Accession Number | BC066245 |
| Gene Symbol | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_3672140 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purfication |
| UNIPROT ID | P68431 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| FC protocol for Acetyl-Histone H3 (Lys4) antibody 84831-1-RR | Download protocol |
| IF protocol for Acetyl-Histone H3 (Lys4) antibody 84831-1-RR | Download protocol |
| WB protocol for Acetyl-Histone H3 (Lys4) antibody 84831-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IF/ICC : 1:500-1:2000 FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension DOT BLOT : 1:10-1:100 CHIP-QPCR : 1:10-1:100 |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 241882E9 |
| Conjugation | Unconjugated |
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