GOT1 Polyclonal antibody 14886-1-AP proteintech
$149.00
In stock
SKU
14886-1-AP
| Catalog Number | 14886-1-AP |
| Synonyms | GIG18, EC:2.6.1.1, cCAT, CAT, cAspAT |
| Host | Rabbit |
| Reactivity | human, mouse, rat and More (1) |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HepG2 cells, L02 cells, mouse brain, rat brain |
| Tested Applications — Positive IP detected in | mouse brain tissue |
| Tested Applications — Positive IF/ICC detected in | L02 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HepG2 cells, L02 cells, mouse brain, rat brain |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | L02 cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag6671 Product name: Recombinant human GOT1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-413 aa of BC000498 Sequence: MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ Predict reactive species |
| Full Name | glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 43-46 kDa |
| GenBank Accession Number | BC000498 |
| Gene Symbol | GOT1 |
| Gene ID (NCBI) | 2805 |
| RRID | AB_2113630 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17174 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for GOT1 antibody 14886-1-AP | Download protocol |
| IP protocol for GOT1 antibody 14886-1-AP | Download protocol |
| WB protocol for GOT1 antibody 14886-1-AP | Download protocol |
| human | WB J Exp Med Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth. Authors - Miao Liu View Article |
| mouse | WB Cell Res Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion. Authors - Wei-Feng Cai KD Validated View Article |
| Daniel (Verified Customer) (04-29-2019) | Strong band of the expected size. Weaker non-specific bands. Applications: Western Blot, Primary Antibody Dilution: 1:1000 Cell Tissue Type: HK2 and RCC4 (human renal cells) GOT1 Antibody Western Blot, validation (1:1000 dilution) in HK2 and RCC4 (human renal cells) (Cat no:14886-1-AP) |
| Citations | 59 |
| Dilutions | WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Glutamate oxaloacetate transaminase 1 (GOT1) catalyzes the reversible reaction of L-aspartate and alpha-ketoglutarate into oxaloacetate and L-glutamate and plays a key role in carbon and nitrogen metabolism. GOT1 can potentially control the intracellular levels of reactive oxygen species (ROS) through NADPH synthesis and enhances tumor growth. GOT1 expression correlates with the growth of several tumors. Protocols Product Specific Protocols IF protocol for GOT1 antibody 14886-1-AP Download protocol IP protocol for GOT1 antibody 14886-1-AP Download protocol WB protocol for GOT1 antibody 14886-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.
Journal: Cell | Authors: Xiulei Mo | Species: human | Application: WB - Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
Journal: Cell Metab | Authors: Qiuying Chen | Application: WB - Glutaminase GLS1 senses glutamine availability in a non-enzymatic manner triggering mitochondrial fusion.
Journal: Cell Res | Authors: Wei-Feng Cai KD Validated | Species: mouse | Application: WB - Sirtuin5 contributes to colorectal carcinogenesis by enhancing glutaminolysis in a deglutarylation-dependent manner.
Journal: Nat Commun | Authors: Yun-Qian Wang | Species: human | Application: WB - Cooperative STAT/NF-κB signaling regulates lymphoma metabolic reprogramming and aberrant GOT2 expression.
Journal: Nat Commun | Authors: Maren Feist | Species: human | Application: WB - Inhibiting both proline biosynthesis and lipogenesis synergistically suppresses tumor growth.
Journal: J Exp Med | Authors: Miao Liu | Species: human | Application: WB
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