B3GNT3 Polyclonal antibody 18098-1-AP proteintech
$149.00
In stock
SKU
18098-1-AP
| Catalog Number | 18098-1-AP |
| Synonyms | Beta-1,3-N-acetylglucosaminyltransferase 3, Beta-1,3-Gn-T3, Beta-1,3-GalTase 8, Beta-1,3-galactosyltransferase 8, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase |
| Host | Rabbit |
| Reactivity | human, mouse |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, mouse lung tissue, SH-SY5Y cells |
| Tested Applications — Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | U2OS cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:500-1:2000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, mouse lung tissue, SH-SY5Y cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag12061 Product name: Recombinant human B3GNT3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 34-372 aa of BC066876 Sequence: CKVQEQPPAIPEALAWPTPPTRPAPAPCHANTSMVTHPDFATQPQHVQNFLLYRHYRHFPLLQDVPPSKCAQPVFLLLVIKSSPSNYVRRELLRRTWGRERKVRGLQLRLLFLVGTASNPHEARKVNRLLELEAQTHGDILQWDFHDSFFNLTLKQVLFLQWQETRCANASFVLNGDDDVFAHTDNMVFYLQDHDPGRHLFVGQLIQNVGPIRAFWSKYYVPEVVTQNERYPPYCGGGGFLLSRFTAAALRRAAHVLDIFPIDDVFLGMCLELEGLKPASHSGIRTSGVRAPSQHLSSFDPCFYRDLLLVHRFLPYEMLLMWDALNQPNLTCGNQTQIY Predict reactive species |
| Full Name | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 |
| Calculated Molecular Weight | 372 aa, 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC066876 |
| Gene Symbol | B3GNT3 |
| Gene ID (NCBI) | 10331 |
| RRID | AB_10596928 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2A9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for B3GNT3 antibody 18098-1-AP | Download protocol |
| IHC protocol for B3GNT3 antibody 18098-1-AP | Download protocol |
| WB protocol for B3GNT3 antibody 18098-1-AP | Download protocol |
| human | IHC Oncol Lett B3GNT3: A prognostic biomarker associated with immune cell infiltration in pancreatic adenocarcinoma. Authors - Kaiwen Kong View Article |
| Citations | 11 |
| Dilutions | WB : 1:500-1:2000 IHC : 1:20-1:200 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Beta-1,3-N-acetylglucosaminyltransferase 3 (B3GNT3) is a type II transmembrane protein located in the Golgi apparatus and contributes to the regulation of L-selectin ligand function, lymphocyte trafficking, and T cell homing. B3GNT3 is overexpressed in breast cancer, and also plays a critical role in the aggressiveness of pancreatic cancer (PMID: 33718036, 30466404). Western blot analysis detected B3GNT3 at an apparent molecular mass of 43 kDa. Protocols Product Specific Protocols IF protocol for B3GNT3 antibody 18098-1-AP Download protocol IHC protocol for B3GNT3 antibody 18098-1-AP Download protocol WB protocol for B3GNT3 antibody 18098-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Eradication of Triple-Negative Breast Cancer Cells by Targeting Glycosylated PD-L1.
Journal: Cancer Cell | Authors: Chia-Wei Li | Species: human | Application: WB - Identifying the prognostic significance of B3GNT3 with PD-L1 expression in lung adenocarcinoma.
Journal: Transl Lung Cancer Res | Authors: Xuefeng Leng | Species: human | Application: IHC - B3GNT3 Expression Is a Novel Marker Correlated with Pelvic Lymph Node Metastasis and Poor Clinical Outcome in Early-Stage Cervical Cancer.
Journal: PLoS One | Authors: Weijing Zhang | Species: human | Application: WB,IHC - A novel glycosylation-related gene signature predicts survival in patients with lung adenocarcinoma
Journal: BMC Bioinformatics | Authors: Jin-Xiao Liang | Species: human | Application: WB - B3GNT3 overexpression is associated with unfavourable survival in non-small cell lung cancer.
Journal: J Clin Pathol | Authors: Liuwei Gao | Species: human | Application: WB,IHC - B3GNT3: A prognostic biomarker associated with immune cell infiltration in pancreatic adenocarcinoma.
Journal: Oncol Lett | Authors: Kaiwen Kong | Species: human | Application: IHC
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