CD147 Polyclonal antibody 11989-1-AP proteintech
$149.00
In stock
SKU
11989-1-AP
| Catalog Number | 11989-1-AP |
| Synonyms | 5F7, Basigin, BSG, Collagenase stimulatory factor, EMMPRIN |
| Host | Rabbit |
| Reactivity | human, mouse and More (1) |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | HeLa cells, human liver tissue, Jurkat cells, mouse heart tissue, mouse liver tissue, HepG2 cells |
| Tested Applications — Positive IP detected in | HEK-293 cells |
| Tested Applications — Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A431 cells, HeLa cells, MCF-7 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | HeLa cells, human liver tissue, Jurkat cells, mouse heart tissue, mouse liver tissue, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells, HeLa cells, MCF-7 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag2619 Product name: Recombinant human CD147 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-269 aa of BC009040 Sequence: MAAALFVLLGFALLGTHGASGAAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALWPFLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS Predict reactive species |
| Full Name | basigin (Ok blood group) |
| Calculated Molecular Weight | 385 aa, 42 kDa |
| Observed Molecular Weight | 35-58 kDa |
| GenBank Accession Number | BC009040 |
| Gene Symbol | CD147 |
| Gene ID (NCBI) | 682 |
| RRID | AB_2290597 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CD147 antibody 11989-1-AP | Download protocol |
| IHC protocol for CD147 antibody 11989-1-AP | Download protocol |
| IP protocol for CD147 antibody 11989-1-AP | Download protocol |
| WB protocol for CD147 antibody 11989-1-AP | Download protocol |
| mouse | CoIP Cell Mol Immunol CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ. Authors - Ruo Chen View Article |
| human | WB Nat Commun Structural insights into the human HRD1 ubiquitin ligase complex. Authors - Liling Guo View Article |
| Mohammed (Verified Customer) (08-19-2020) | Stains the sperm tail very nicely. Applications: Immunofluorescence Primary Antibody Dilution: (1:200) Cell Tissue Type: Testis |
| Citations | 55 |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:400-1:1600 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion and an extracellular portion and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020). The CD147 gene, designated BSG for basigin, is located on chromosome 19p13.3. Four transcript variants encoding different isoforms have been found (PMID: 21536654). CD147 migrates on SDS-PAGE usually between 35 and 65 kDa, depending on the degree of glycosylation (PMID: 17945211). Protocols Product Specific Protocols IF protocol for CD147 antibody 11989-1-AP Download protocol IHC protocol for CD147 antibody 11989-1-AP Download protocol IP protocol for CD147 antibody 11989-1-AP Download protocol WB protocol for CD147 antibody 11989-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- CD147 deficiency in T cells prevents thymic involution by inhibiting the EMT process in TECs in the presence of TGFβ.
Journal: Cell Mol Immunol | Authors: Ruo Chen | Species: mouse | Application: CoIP - Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles
Journal: J Extracell Vesicles | Authors: Liqiao Hu | Species: human | Application: WB - Secretory autophagy mediates SLC16A3/MCT4-dependent lactate secretion to drive metastatic progression in triple-negative breast cancer.
Journal: Autophagy | Authors: Shan-Ying Wu | Species: human | Application: WB - SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex
Journal: Nat Commun | Authors: Liangguang Leo Lin | Species: human | Application: WB - Structural basis and pathological implications of the dimeric OS9-SEL1L-HRD1 ERAD Core Complex.
Journal: Nat Commun | Authors: Liangguang Leo Lin | Species: human | Application: WB - Structural insights into the human HRD1 ubiquitin ligase complex.
Journal: Nat Commun | Authors: Liling Guo | Species: human | Application: WB - Structural basis and pathological implications of the dimeric OS9-SEL1L-HRD1 ERAD Core Complex.
Journal: Nat Commun | Authors: Liangguang Leo Lin | Species: human | Application: WB - SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex
Journal: Nat Commun | Authors: Liangguang Leo Lin | Species: human | Application: WB - Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders
Journal: J Clin Invest | Authors: Huilun Helen Wang | Species: human | Application: WB - Biallelic Cys141Tyr variant of SEL1L is associated with neurodevelopmental disorders, agammaglobulinemia, and premature death
Journal: J Clin Invest | Authors: Denisa Weis | Species: human | Application: WB - β-Elemene Rescues Radiation-Induced Enteritis by Orchestrating a Host-Microbiome Circuit That Fuels Epigenetic DNA Repair.
Journal: Adv Sci (Weinh) | Authors: Jiancheng He | Species: mouse | Application: IHC,IF - A CD147-targeted small-molecule inhibitor potentiates gemcitabine efficacy by triggering ferroptosis in pancreatic ductal adenocarcinoma
Journal: Cell Rep Med | Authors: Jiaqi Li - Alarmin S100A8 imparts chemoresistance of esophageal cancer by reprogramming cancer-associated fibroblasts
Journal: Cell Rep Med | Authors: Xinjie Chen | Species: human | Application: WB - Fyn inhibition by TAE684: A synergistic strategy to suppress melanoma and reverse vemurafenib resistance.
Journal: Cell Death Dis | Authors: Waner Liu | Species: human | Application: WB - The mechanism of extracellular CypB promotes glioblastoma adaptation to glutamine deprivation microenvironment
Journal: Cancer Lett | Authors: Hang Yin | Species: human | Application: WB,IP - 124I-labeled anti-CD147 antibody for noninvasive detection of CD147-positive pan-cancers: construction and preclinical studies
Journal: Acta Pharmacol Sin | Authors: Xiao-Kun Ma | Species: mouse | Application: IHC - Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.
Journal: EMBO J | Authors: Huilun Helen Wang | Species: mouse | Application: WB - Large-Scale Single-Cell and Bulk Sequencing Analyses Reveal the Prognostic Value and Immune Aspects of CD147 in Pan-Cancer.
Journal: Front Immunol | Authors: Jingwei Zhang | Species: human | Application: IF - TanGIBLE: A selective probe for evaluating hydrophobicity-exposed defective proteins in live cells
Journal: J Cell Biol | Authors: Yasuyuki Iwasa - Arf1 is involved in Neisseria meningitidis-induced cortical branched F-actin network reorganization.
Journal: EMBO Rep | Authors: Margot Sahnine - Lactate Activates AMPK Remodeling of the Cellular Metabolic Profile and Promotes the Proliferation and Differentiation of C2C12 Myoblasts
Journal: Int J Mol Sci | Authors: Yu Zhou | Species: mouse | Application: WB - CD147 mediates the adsorption of influenza A virus on the cell surface through direct interaction with HA.
Journal: Front Cell Infect Microbiol | Authors: Ting Wang | Species: human | Application: WB,CoIP - α2,6-Sialylation promotes immune escape in hepatocarcinoma cells by regulating T cell functions and CD147/MMP signaling.
Journal: J Physiol Biochem | Authors: Liping Wang | Species: human | Application: WB - CD147 promotes IKK/IκB/NF-κB pathway to resist TNF-induced apoptosis in rheumatoid arthritis synovial fibroblasts.
Journal: J Mol Med (Berl) | Authors: Yue Zhai | Species: human | Application: WB - Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication.
Journal: PLoS Pathog | Authors: Tao Wang | Species: human | Application: WB - UBE2C-Mediated CD147-CTLA-4 Axis Promotes T Cell Exhaustion and Immunosuppressive Microenvironment in Bladder Cancer.
Journal: Cancer Sci | Authors: Xuwei Hong | Species: mouse | Application: WB - Novel long non-coding RNA CYB561-5 promotes aerobic glycolysis and tumorigenesis by interacting with basigin in non-small cell lung cancer.
Journal: J Cell Mol Med | Authors: Longfei Li | Species: human | Application: WB - Serum Exosomal mir-340-5p Promotes Angiogenesis in Brain Microvascular Endothelial Cells During Oxygen-Glucose Deprivation.
Journal: Neurochem Res | Authors: Chuan Xu | Species: mouse | Application: WB - SLC16a6, mTORC1, and Autophagy Regulate Ketone Body Excretion in the Intestinal Cells
Journal: Biology (Basel) | Authors: Takashi Uebanso | Species: human | Application: WB - Autophagy Induced by Glycosylated CD147 Attenuates Fibroblast Activation and Myofibroblast Differentiation.
Journal: J Biol Chem | Authors: Siqi Long | Species: human, mouse | Application: IHC,IF - Heat shock protein A12A activates migration of hepatocellular carcinoma cells in a monocarboxylate transporter 4-dependent manner.
Journal: Cell Stress Chaperones | Authors: Xinxu Min | Species: human | Application: IP - Combination of selenium and metformin mitigated testicular damage from diabetes mellitus by enhancing glycolysis via AMPK/ SIRT1 pathway.
Journal: J Trace Elem Med Biol | Authors: Li Cao | Species: mouse | Application: WB,IF - Requirements for nuclear GRP78 transcriptional regulatory activities and interaction with nuclear GRP94
Journal: J Biol Chem | Authors: Ze Liu | Application: WB - Autophagy Induced by Glycosylated CD147 Attenuates Fibroblast Activation and Myofibroblast Differentiation.
Journal: J Biol Chem | Authors: Siqi Long | Species: human, mouse | Application: IHC,IF - LncRNA RHPN1-AS1 accelerates proliferation, migration, and invasion via regulating miR-485-5p/BSG axis in hepatocellular carcinoma.
Journal: Naunyn Schmiedebergs Arch Pharmacol | Authors: Wei Zhang | Species: human | Application: WB - Resveratrol-induced apoptosis is associated with regulating the miR-492/CD147 pathway in malignant melanoma cells.
Journal: Naunyn Schmiedebergs Arch Pharmacol | Authors: Shuang Zhao | Species: human | Application: WB - Changes in Corticotrope Gene Expression Upon Increased Expression of Peptidylglycine α-Amidating Monooxygenase.
Journal: Endocrinology | Authors: Richard E Mains | Species: mouse | Application: WB - Association of increased ligand cyclophilin A and receptor CD147 with hypoxia, angiogenesis, metastasis and prognosis of tongue squamous cell carcinoma.
Journal: Histopathology | Authors: Huang Congfa C | Species: human | Application: IHC - A chimeric antibody targeting CD147 inhibits hepatocellular carcinoma cell motility via FAK-PI3K-Akt-Girdin signaling pathway.
Journal: Clin Exp Metastasis | Authors: Yuan Wang | Species: mouse | Application: IHC - Interaction of CD147 and human epididymis protein 4 promotes invasion and metastasis of ovarian cancer.
Journal: J Cancer | Authors: Lingling Gao | Species: human | Application: WB,IHC,CoIP - Cytoskeletal β-tubulin and cysteine cathepsin L deregulation by SARS-CoV-2 spike protein interaction with the neuronal model cell line SH-SY5Y
Journal: Biochimie | Authors: Bernardo R Oliveira | Species: human | Application: IF - Effects of excess thromboxane A2 on placental development and nutrient transporters in a Mus musculus model of fetal growth restriction.
Journal: Biol Reprod | Authors: Karen J Gibbins - Inhibition of STAT3 reduces proliferation and invasion in salivary gland adenoid cystic carcinoma.
Journal: Am J Cancer Res | Authors: Lin-Lin Bu | Species: human | Application: IHC - C4.4A as a biomarker of head and neck squamous cell carcinoma and correlated with epithelial mesenchymal transition.
Journal: Am J Cancer Res | Authors: Jian-Feng Liu | Species: human | Application: IHC,IF - Evaluating Basigin as a Potential Biomarker of Blood-Brain Barrier Dysfunction in Cerebral Amyloid Angiopathy.
Journal: Neuropathol Appl Neurobiol | Authors: Arno Stellingwerf | Species: human | Application: IHC - Clinical significance of keap1 and nrf2 in oral squamous cell carcinoma.
Journal: PLoS One | Authors: Cong-Fa Huang | Species: human | Application: IHC - FGF2 as a potential prognostic biomarker for proneural glioma patients.
Journal: Acta Oncol | Authors: Linda Sooman | Species: human | Application: IHC - Intervention with extracellular matrix metalloproteinase inducer in osteoclasts attenuates periodontitis-induced bone resorption
Journal: Odontology | Authors: Yuxin Wang | Species: rat | Application: WB,IHC,IF - CD147 inhibition reduced fibronectin expression in TGF-β1-induced keloid fibroblasts by targeting Smad2 signaling pathway
Journal: Burns | Authors: Jian-Sheng Diao | Species: human | Application: WB,IHC,IF - Triptolide inhibits the migration and invasion of human prostate cancer cells via Caveolin-1/CD147/MMPs pathway.
Journal: Biomed Pharmacother | Authors: Shiqi Yuan | Species: human | Application: WB - Association Study between an SNP in CD147 and Its Expression With Acute Coronary Syndrome in a Jiangsu Chinese Population.
Journal: Medicine (Baltimore) | Authors: Jinchuan Yan | Species: human | Application: WB - The fucosylated CD147 enhances the autophagy in epithelial ovarian cancer cells.
Journal: Oncotarget | Authors: Zhenhua Hu | Application: IP - Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes
Journal: Elife | Authors: Abhishek Jamwal | Species: human | Application: WB - CD44 cross-linking promotes Plasmodium falciparum invasion.
Journal: bioRxiv | Authors: Angel K. Kongsomboonvech | Species: human - Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes
Journal: Elife | Authors: Abhishek Jamwal | Species: human | Application: WB - CD147-SNAP-tag-CMC/HPLC-MS two-dimensional coupling system: a universal platform for screening active components targeting CD147 for anti-liver cancer applications.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci | Authors: Wenhao Chen | Species: human | Application: WB,IF
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