BCKDK Polyclonal antibody 15718-1-AP proteintech
$149.00
In stock
SKU
15718-1-AP
| Catalog Number | 15718-1-AP |
| Synonyms | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial, BCKDH kinase, BCKDHKIN, BDK, Branched-chain alpha-ketoacid dehydrogenase kinase |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse kidney tissue, HEK-293 cells, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue |
| Tested Applications — Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:6000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | mouse kidney tissue, HEK-293 cells, mouse pancreas tissue, rat kidney tissue, rat pancreas tissue |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag8395 Product name: Recombinant human BCKDK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 64-412 aa of BC007363 Sequence: EKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI Predict reactive species |
| Full Name | branched chain ketoacid dehydrogenase kinase |
| Calculated Molecular Weight | 412 aa, 46 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC007363 |
| Gene Symbol | BCKDK |
| Gene ID (NCBI) | 10295 |
| RRID | AB_2878174 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14874 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for BCKDK antibody 15718-1-AP | Download protocol |
| IHC protocol for BCKDK antibody 15718-1-AP | Download protocol |
| WB protocol for BCKDK antibody 15718-1-AP | Download protocol |
| mouse | WB Biochim Biophys Acta Mol Basis Dis Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury Authors - Xiao Han View Article |
| human | WB J Exp Clin Cancer Res Acetylation-induced degradation of ECHS1 enhances BCAA accumulation and proliferation in KRAS-mutant colorectal cancer Authors - Zhenkang Li View Article |
| Citations | 3 |
| Dilutions | WB : 1:1000-1:6000 IHC : 1:200-1:800 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
Publications
- Pyridostigmine Protects Against Diabetic Cardiomyopathy by Regulating Vagal Activity, Gut Microbiota, and Branched-Chain Amino Acid Catabolism in Diabetic Mice.
Journal: Front Pharmacol | Authors: Yang Yang | Species: mouse | Application: WB - Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury
Journal: Biochim Biophys Acta Mol Basis Dis | Authors: Xiao Han | Species: mouse | Application: WB - Acetylation-induced degradation of ECHS1 enhances BCAA accumulation and proliferation in KRAS-mutant colorectal cancer
Journal: J Exp Clin Cancer Res | Authors: Zhenkang Li | Species: human | Application: WB
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