BHLHE41 Polyclonal antibody 12688-1-AP proteintech
$149.00
In stock
SKU
12688-1-AP
| Catalog Number | 12688-1-AP |
| Synonyms | BHLHB3, Class B basic helix-loop-helix protein 3, Class E basic helix-loop-helix protein 41, DEC2, Enhancer-of-split and hairy-related protein 1 |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, ChIP, ELISA |
| Applications | WB, IHC, IF/ICC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | mouse testis tissue, rat testis tissue |
| Tested Applications — Positive IHC detected in | human ovary tumor tissue, mouse brain tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | A431 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:12000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human ovary tumor tissue, mouse brain tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3381 Product name: Recombinant human BHLHE41 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC025968 Sequence: MDEGIPHLQERQLLEHRDFIGLDYSSLYMCKPKRSMKRDDTKDTYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLKHLKALTALTEQQHQKIIALQNGERSLKSPIQSDLDAFHSGFQTCAKEVLQYLSRFESWTPREPRCVQLINHLHAVATQFLPTPQLLTQQVPLSKGTGAPSAAGSAAAPCLERAGQKLEPLAYCVPVIQRTQPSAELAAENDTDTDSGYGGEAEARPDREKGKGAGASRVTIKQEPPGEDSPAPKRMKLDSRGGGSGGGPGGGAVAAAAALLGPDPAAAAALLRPDAALLSSLVAFGGGGGAPFPQPAAAAAPFCLPFC Predict reactive species |
| Full Name | basic helix-loop-helix family, member e41 |
| Calculated Molecular Weight | 482 aa, 51 kDa |
| Observed Molecular Weight | 50-52 kDa |
| GenBank Accession Number | BC025968 |
| Gene Symbol | BHLHE41 |
| Gene ID (NCBI) | 79365 |
| RRID | AB_2065361 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C0J9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for BHLHE41 antibody 12688-1-AP | Download protocol |
| IHC protocol for BHLHE41 antibody 12688-1-AP | Download protocol |
| WB protocol for BHLHE41 antibody 12688-1-AP | Download protocol |
| human | WB,IF Cell Death Dis Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells. Authors - Meng Zhang View Article |
| rat | WB FASEB J Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin. Authors - Annette Schröder View Article |
| Citations | 13 |
| Dilutions | WB : 1:2000-1:12000 IHC : 1:2000-1:8000 IF/ICC : 1:50-1:500 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
BHLHE41 (basic helix-loop-helix family member e41), also called SHARP1 and DEC2, is a member of the BHLH family proteins that are involved in differentiation, circadian rhythms and the response to hypoxia. In esophageal, osteosarcomas, prostate, breast, and endometrial cancer cells, BHLHE41 functions as an anti-apoptotic factor and promotes cell proliferation and invasiveness. It suppresses cell proliferation and metastasis, and also facilitates pancreatic as well as gastric cancer cell apoptosis. BHLHE41 has an important role in the proliferation of glioblastoma and couldserve as a novel candidate for targeted therapy of glioblastoma (PMID: 31616182). Protocols Product Specific Protocols IF protocol for BHLHE41 antibody 12688-1-AP Download protocol IHC protocol for BHLHE41 antibody 12688-1-AP Download protocol WB protocol for BHLHE41 antibody 12688-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 acute myelogenous leukemia.
Journal: Nat Commun | Authors: Akihiko Numata | Species: human | Application: chIP - Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin.
Journal: FASEB J | Authors: Annette Schröder | Species: rat | Application: WB - Loss of circadian protein TIMELESS accelerates the progression of cellular senescence.
Journal: Biochem Biophys Res Commun | Authors: Xiaomeng Shen | Species: human | Application: WB - DEC2 expression is positively correlated with HIF-1 activation and the invasiveness of human osteosarcomas.
Journal: J Exp Clin Cancer Res | Authors: Tu Hu | Species: human | Application: WB,IHC - Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma.
Journal: J Exp Clin Cancer Res | Authors: Xiao Yang | Species: human | Application: WB,IHC - Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.
Journal: Cell Death Dis | Authors: Meng Zhang | Species: human | Application: WB,IF - The Megacomplex protects ER-alpha from degradation by Fulvestrant in epithelial ovarian cancer
Journal: Cancer Lett | Authors: Sushil Kumar Jaiswal | Species: human | Application: WB - Diurnal Expression of PD-1 on Tumor-Associated Macrophages Underlies the Dosing Time-Dependent Antitumor Effects of the PD-1/PD-L1 Inhibitor BMS-1 in B16/BL6 Melanoma-Bearing Mice
Journal: Mol Cancer Res | Authors: Akito Tsuruta | Species: mouse | Application: WB - Hypoxia induced cell dormancy of salivary adenoid cystic carcinoma through miR-922/DEC2 axis
Journal: Transl Oncol | Authors: Li Dai | Species: human | Application: WB,IHC - Persistent myopathy despite release of partial obstruction: in vivo reversal of dysfunction and transcriptional responses using rapamycin.
Journal: FASEB J | Authors: Annette Schröder | Species: rat | Application: WB - Cancer-associated fibroblasts-derived CXCL1 activates DEC2-mediated dormancy in oral squamous cell carcinoma
Journal: Heliyon | Authors: Wei-Long Zhang | Application: WB,IHC - Deciphering the immunological landscape of HR + metastatic breast cancer: insights from single-cell transcriptomics.
Journal: Hum Cell | Authors: Lifang He | Species: human | Application: IHC - Loss of circadian protein TIMELESS accelerates the progression of cellular senescence.
Journal: Biochem Biophys Res Commun | Authors: Xiaomeng Shen | Species: human | Application: WB
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