BHMT Recombinant monoclonal antibody 86372-1-RR proteintech
$149.00
In stock
SKU
86372-1-RR
| Catalog Number | 86372-1-RR |
| Synonyms | Betaine--homocysteine S-methyltransferase 1, EC:2.1.1.5 |
| Host | Rabbit |
| Reactivity | human, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | rat liver tissue, human kidney tissue |
| Tested Applications — Positive IP detected in | rat liver tissue |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Positive WB detected in | rat liver tissue, human kidney tissue |
| Positive IP detected in | rat liver tissue |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Tested Reactivity | human, rat |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | CatNo: Ag8738 Product name: Recombinant human BHMT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-406 aa of BC012616 Sequence: MPPVGGKKAKKGILERLNAGEIVIGDGGFVFALEKRGYVKAGPWTPEAAVEHPEAVRQLHREFLRAGSNVMQTFTFYASEDKLENRGNYVLEKISGQEVNEAACDIARQVADEGDALVAGGVSQTPSYLSCKSETEVKKVFLQQLEVFMKKNVDFLIAEYFEHVEEAVWAVETLIASGKPVAATMCIGPEGDLHGVPPGECAVRLVKAGASIIGVNCHFDPTISLKTVKLMKEGLEAARLKAHLMSQPLAYHTPDCNKQGFIDLPEFPFGLEPRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWVRARARKEYWENLRIASGRPYNPSMSKPDGWGVTKGTAELMQQKEATTEQQLKELFEKQKFKSQ Predict reactive species |
| Full Name | betaine-homocysteine methyltransferase |
| Calculated Molecular Weight | 406 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC012616 |
| Gene Symbol | BHMT |
| Gene ID (NCBI) | 635 |
| RRID | AB_3744848 |
| Conjugate | Unconjugated |
| Purification Method | Protein A purification |
| UNIPROT ID | Q93088 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IP protocol for BHMT antibody 86372-1-RR | Download protocol |
| WB protocol for BHMT antibody 86372-1-RR | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB |
| Isotype | IgG |
| Clonality | Recombinant |
| Clone Number | 250978E3 |
| Conjugation | Unconjugated |
Betaine-homocysteine methyltransferase (BHMT) is a cytosolic enzyme that Involved in the regulation of homocysteine metabolism. BHMT catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline(PMID: 8798461, 10529246). Protocols Product Specific Protocols IP protocol for BHMT antibody 86372-1-RR Download protocol WB protocol for BHMT antibody 86372-1-RR Download protocol Standard Protocols Click here to view our Standard Protocols
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