| Catalog Number | 60971-1-Ig |
| Synonyms | Bromodomain-containing protein 3, KIAA0043, ORFX, RING3 like protein, RING3L |
| Host | Mouse |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, ELISA |
| Host / Isotype | Mouse / IgG1 |
| Tested Applications — Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, HSC-T6 cells, NIH/3T3 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:5000-1:50000 |
| Positive WB detected in | LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, MOLT-4 cells, HSC-T6 cells, NIH/3T3 cells |
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Tested Reactivity | human, mouse, rat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag33684 Product name: Recombinant human BRD3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-400 aa of BC032124 Sequence: MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAM Predict reactive species |
| Full Name | bromodomain containing 3 |
| Calculated Molecular Weight | 556 aa, 61 kDa |
| Observed Molecular Weight | 95-105 kDa |
| GenBank Accession Number | BC032124 |
| Gene Symbol | BRD3 |
| Gene ID (NCBI) | 8019 |
| RRID | AB_3743256 |
| Conjugate | Unconjugated |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| WB protocol for BRD3 antibody 60971-1-Ig | Download protocol |
| Citations | - |
| Dilutions | WB : 1:5000-1:50000 |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Clone Number | 1C8C11 |
| Conjugation | Unconjugated |
BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123). Protocols Product Specific Protocols WB protocol for BRD3 antibody 60971-1-Ig Download protocol Standard Protocols Click here to view our Standard Protocols
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