HRP-conjugated Beta Actin Monoclonal antibody HRP-60008 proteintech
$189.00
In stock
SKU
HRP-60008
| Catalog Number | HRP-60008 |
| Synonyms | Actin, ACTB, bate actin, β actin, Beta-actin |
| Host | Mouse |
| Reactivity | human, mouse, rat, zebrafish, plant and More (6) |
| Reactivity | human, mouse, rat, zebrafish, plant, pig, canine, monkey, chicken, hamster, goat |
| Form | Liquid |
| Formulation | PBS, Proclin300, BSA, Glycerol |
| Applications | WB, IF, ELISA |
| Applications | WB |
| Host / Isotype | Mouse / IgM |
| Tested Applications — Positive WB detected in | HeLa cells, multi-cells/tissue, HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:2000-1:16000 |
| Positive WB detected in | HeLa cells, multi-cells/tissue, HepG2 cells |
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Tested Reactivity | human, mouse, rat, zebrafish, plant |
| Cited Reactivity | human, mouse, rat, pig, canine, monkey, chicken, hamster, goat |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant protein Predict reactive species |
| Full Name | actin, beta |
| Calculated Molecular Weight | 375 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC002409 |
| Gene Symbol | Beta Actin |
| Gene ID (NCBI) | 60 |
| RRID | AB_2819183 |
| Conjugate | HRP |
| Purification Method | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | P60709 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. |
| WB protocol for HRP Beta Actin antibody HRP-60008 | Download protocol |
| human | WB Cell Mol Immunol The NET-DNA-CCDC25 inhibitor di-Pal-MTO suppresses tumor progression and promotes the innate immune response Authors - Shun Wang View Article |
| mouse | WB Blood Fetal liver hepcidin secures iron stores in utero. Authors - Lara Kämmerer View Article |
| Robert (Verified Customer) (09-24-2025) | You most likely need less than you think you do. Otherwise bright and great. Gets the job done fast and efficiently Applications: Western Blot Primary Antibody Dilution: 1:10000 Cell Tissue Type: HCEC |
| Dhanwini (Verified Customer) (09-24-2025) | GOOD Applications: Western Blot |
| Sneha (Verified Customer) (09-24-2025) | GOOD Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Primary Antibody Dilution: 1:1000 Cell Tissue Type: ARPE-19 |
| Michael (Verified Customer) (09-18-2025) | Works really well with western blots Applications: Western Blot, Immunofluorescence, Cell culture Primary Antibody Dilution: 1:5000 Cell Tissue Type: Corneal cells |
| Robert (Verified Customer) (09-02-2025) | Works exactly as intended. You need less than you think. Applications: Western Blot Primary Antibody Dilution: 1:10000 |
| Henry (Verified Customer) (09-01-2025) | This is very good and reduces the burden of secondary antibodies Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: Muller glia, bmdm, neutrophils, PBMCs |
| Sara (Verified Customer) (10-05-2024) | I love Proteintech HRP-conjugated antibodies. These are def my favourites for WB controls (housekeeping proteins), I have got actin, tubulin and GAPDH. Very good bands, super reliable. Applications: Western Blot Primary Antibody Dilution: 1:10.000 Cell Tissue Type: Mouse |
| Maria (Verified Customer) (10-24-2023) | Used Incubation over night at 4°C, never tested a shorter incubation, but it worked very nice. Applications: Western Blot Primary Antibody Dilution: 1/5000 Cell Tissue Type: whole lysate in UREA made from muscles, brown fat and white fat. species: mice and naked molerats |
| Priya (Verified Customer) (06-21-2023) | Used this antibody for Caco2 cells andmice tissue Applications: Western Blot Primary Antibody Dilution: 1 to 1000 Cell Tissue Type: Caco2, Mice colon |
| Wojciech (Verified Customer) (04-20-2023) | The beta actin works very well diluted at 1:5000. It was used on T-ALL cell lines. Loading 30 ug of whole protein lysate. Applications: Western Blot Primary Antibody Dilution: 1:5000 Cell Tissue Type: T cells |
| Priya (Verified Customer) (04-17-2023) | Used for Caco2 cells Applications: Western Blot Primary Antibody Dilution: 1 to 5000 Cell Tissue Type: Caco2, Mice colon |
| Iru (Verified Customer) (06-07-2021) | The antibody works well. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: Fallopian tube epithelial cells |
| Azita (Verified Customer) (06-02-2021) | Western blot analysis using HRP- conjugated beta actin in NSC34 cell line at dilution of 1:10000 Applications: Western Blot Primary Antibody Dilution: 1:10000 Cell Tissue Type: NSC34 cells HRP Beta Actin Antibody Western Blot validation (1:10000 dilution) in NSC34 cells (Cat no:HRP-60008) |
| Hasibur (Verified Customer) (08-01-2019) | Nice antibody and no need for secondary antibody Applications: Western Blot, Primary Antibody Dilution: 1:20000 Cell Tissue Type: Bladder Cancer |
| Joanne (Verified Customer) (01-22-2019) | Tested using 10ug of Caco-2 cell lysate and whole membrane. A strong band with no background and a great time saver! Applications: Western Blot, Primary Antibody Dilution: 1:5000 Cell Tissue Type: caco-2 cell HRP Beta Actin Antibody Western Blot, validation (1:5000 dilution) in caco-2 cell (Cat no:HRP-60008) |
| Tess (Verified Customer) (11-29-2018) | Little to no background, and takes half the time. Applications: Western Blot, Primary Antibody Dilution: 1:10,000 Cell Tissue Type: human colorectal cancer cell line HRP Beta Actin Antibody Western Blot, validation (1:10,000 dilution) in human colorectal cancer cell line (Cat no:HRP-60008) |
| Citations | 568 |
| Dilutions | WB : 1:2000-1:16000 |
| Isotype | IgM |
| Clonality | Monoclonal |
| Clone Number | 7D2C10 |
| Conjugation | HRP |
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP. Protocols Product Specific Protocols WB protocol for HRP Beta Actin antibody HRP-60008 Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate
Journal: Cell | Authors: Kazushige Shiraishi | Application: WB - Dual inhibition of CDK4/6 and XPO1 induces senescence with acquired vulnerability to CRBN-based PROTAC drugs
Journal: Gastroenterology | Authors: Hui Wang - METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation
Journal: J Hematol Oncol | Authors: Meilin Xue | Application: WB - Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.
Journal: Nat Neurosci | Authors: Kornélia Szebényi | Application: WB - The NET-DNA-CCDC25 inhibitor di-Pal-MTO suppresses tumor progression and promotes the innate immune response
Journal: Cell Mol Immunol | Authors: Shun Wang | Species: human | Application: WB - Fetal liver hepcidin secures iron stores in utero.
Journal: Blood | Authors: Lara Kämmerer | Species: mouse | Application: WB
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