TRPA1 Polyclonal antibody 19124-1-AP proteintech
$149.00
In stock
SKU
19124-1-AP
| Catalog Number | 19124-1-AP |
| Synonyms | ANKTM1, p120, Wasabi receptor |
| Host | Rabbit |
| Reactivity | human, mouse, rat, pig |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF, ELISA |
| Applications | WB, IHC, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | SH-SY5Y cells, SK-N-SH cells, pig colon tissue |
| Tested Applications — Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue, rat dorsal root ganglion tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Positive WB detected in | SH-SY5Y cells, SK-N-SH cells, pig colon tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue, rat dorsal root ganglion tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide Predict reactive species |
| Full Name | transient receptor potential cation channel, subfamily A, member 1 |
| Calculated Molecular Weight | 127 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_007332 |
| Gene Symbol | TRPA1 |
| Gene ID (NCBI) | 8989 |
| RRID | AB_10642143 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75762 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IHC protocol for TRPA1 antibody 19124-1-AP | Download protocol |
| WB protocol for TRPA1 antibody 19124-1-AP | Download protocol |
| mouse | WB,IF Mol Nutr Food Res Menthol from Mentha piperita Suppresses the Milk Production of Lactating Mammary Epithelial Cells In Vivo and In Vitro. Authors - Norihiro Suzuki View Article |
| rat | WB Phytomedicine Yu-Xue-Bi capsule ameliorates aggressive synovitis and joint damage in rheumatoid arthritis via modulating the SUCNR1/HIF-1α/TRPV1 axis Authors - Wenjia Chen View Article |
| rat,human | WB,IF J Ethnopharmacol Ligustilide covalently binds to Cys703 in the pre-S1 helix of TRPA1, blocking the opening of channel and relieving pain in rats with acute soft tissue injury Authors - Kaixue Zhang View Article |
| Citations | 45 |
| Dilutions | WB : 1:1000-1:4000 IHC : 1:250-1:1000 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
TRPA1, also named as ANKTM1, belongs to the transient receptor family. TRPA1 is a receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. TRPA1 has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. It acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. It may be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Protocols Product Specific Protocols IHC protocol for TRPA1 antibody 19124-1-AP Download protocol WB protocol for TRPA1 antibody 19124-1-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- IFT80 and TRPA1 cooperatively regulate bone formation by calcium signaling in response to mechanical stimuli
Journal: Metabolism | Authors: Ting Wang | Species: mouse | Application: WB,IF - Yu-Xue-Bi capsule ameliorates aggressive synovitis and joint damage in rheumatoid arthritis via modulating the SUCNR1/HIF-1α/TRPV1 axis
Journal: Phytomedicine | Authors: Wenjia Chen | Species: rat | Application: WB - A dual receptors-targeting and size-switchable "cluster bomb" co-loading chemotherapeutic and transient receptor potential ankyrin 1 (TRPA-1) inhibitor for treatment of triple negative breast cancer.
Journal: J Control Release | Authors: Yashi Wang | Species: mouse | Application: IHC - The efficacy of ophiopogonanone B in treating the cough in mice infected with Mycoplasma pneumoniae
Journal: Front Pharmacol | Authors: Nan-Nan Liu - Ligustilide covalently binds to Cys703 in the pre-S1 helix of TRPA1, blocking the opening of channel and relieving pain in rats with acute soft tissue injury
Journal: J Ethnopharmacol | Authors: Kaixue Zhang | Species: rat,human | Application: WB,IF - Menthol from Mentha piperita Suppresses the Milk Production of Lactating Mammary Epithelial Cells In Vivo and In Vitro.
Journal: Mol Nutr Food Res | Authors: Norihiro Suzuki | Species: mouse | Application: WB,IF - TRPA1 promotes overactive bladder progression by activating the NLRP3 inflammasome and driving pyroptosis.
Journal: Cell Death Dis | Authors: Yongjuan Rao | Species: rat,mouse,human | Application: WB - Cannabidiol (CBD): a killer for inflammatory rheumatoid arthritis synovial fibroblasts.
Journal: Cell Death Dis | Authors: Torsten Lowin | Species: human - Yu-Xue-Bi capsule ameliorates aggressive synovitis and joint damage in rheumatoid arthritis via modulating the SUCNR1/HIF-1α/TRPV1 axis
Journal: Phytomedicine | Authors: Wenjia Chen | Species: rat | Application: WB - Electroacupuncture reduces calcium influx and inhibits endoplasmic reticulum stress by inhibiting the PKCδ-TRPA1 pathway to promote motor function recovery after spinal cord injury.
Journal: Burns Trauma | Authors: Yihui Zhang - Elevated bile acids induce circadian rhythm sleep disorders in chronic liver diseases
Journal: Cell Mol Gastroenterol Hepatol | Authors: Lan Zhou | Species: mouse | Application: WB - Mechanism Study on Liquiritin Alleviating Nociceptive Sensitization in Knee Osteoarthritis via Promoting M2 Macrophage Polarization Through Regulation of the Rap1/PI3K/Akt Signaling Pathway.
Journal: Phytother Res | Authors: Hou-Yu Fu | Species: mouse | Application: WB,IF - Dissecting the effect of ileal faecal diversion on the intestine using single-cell sequencing
Journal: Clin Transl Med | Authors: Haitao Ma | Species: mouse | Application: WB,IHC - Aconitine - A promising candidate for treating cold and mechanical allodynia in cancer induced bone pain
Journal: Biomed Pharmacother | Authors: Xiang Jin | Species: mouse | Application: WB - Euphorbia pekinensis glycosphingolipids disrupt gut motility and fluid balance via TRPA1 activation in enterochromaffin cells.
Journal: Br J Pharmacol | Authors: Jie Cao | Species: mouse | Application: IF - Casticin promotes M2 macrophage polarisation and dorsal root ganglion neuronal autophagy to alleviate cold hyperalgesia in knee osteoarthritis by regulating histone-lysine N-methyltransferase (SETDB2).
Journal: Br J Pharmacol | Authors: Yibao Wei - High humidity and NO2 co-exposure exacerbates allergic asthma by increasing oxidative stress, inflammatory and TRP protein expressions in lung tissue
Journal: Environ Pollut | Authors: Chan Lu | Species: mouse | Application: IHC - Zhisou powder suppresses airway inflammation in LPS and CS-induced post-infectious cough model mice via TRPA1/TRPV1 channels
Journal: J Ethnopharmacol | Authors: Yuan Xu | Species: mouse | Application: WB - Water extract of Notopterygium incisum alleviates cold allodynia in neuropathic pain by regulation of TRPA1
Journal: J Ethnopharmacol | Authors: Yonglan Ruan | Species: mouse | Application: WB,IF - Ligustilide improves functional constipation by non-covalently activating TRPA1 in colon tissue
Journal: J Ethnopharmacol | Authors: Kaixue Zhang | Species: mouse,human | Application: WB,IF - Ligustilide covalently binds to Cys703 in the pre-S1 helix of TRPA1, blocking the opening of channel and relieving pain in rats with acute soft tissue injury
Journal: J Ethnopharmacol | Authors: Kaixue Zhang | Species: rat,human | Application: WB,IF - Bromelain Alleviates Sleep Deprivation-Induced Intestinal Injury via TRPA1/5-HT Axis
Journal: J Agric Food Chem | Authors: Hai-Yi Zhang | Species: mouse | Application: WB,IF - Protein phosphatase 2Cm-regulated branched-chain amino acid catabolic defect in dorsal root ganglion neurons drives pain sensitization
Journal: Acta Neuropathol Commun | Authors: Nan Lian | Species: mouse | Application: WB - A novel technique of cryodenervation for murine vagus nerve: implications for acute lung inflammation
Journal: Respir Res | Authors: Di Wu | Species: mouse | Application: WB,IF - Role of TRPV1 and TRPA1 in TSLP production in nasal epithelial cells
Journal: Int Immunopharmacol | Authors: Jiani Li | Species: human | Application: IF - The efficacy of ophiopogonanone B in treating the cough in mice infected with Mycoplasma pneumoniae
Journal: Front Pharmacol | Authors: Nan-Nan Liu - Propofol exerts neuroprotective functions by down-regulating microRNA-19a in glutamic acid-induced PC12 cells.
Journal: Biofactors | Authors: Shashuang Yu | Species: mouse | Application: WB,IHC - Effect of β-hydroxybutyrate on behavioral alterations, molecular and morphological changes in CNS of multiple sclerosis mouse model
Journal: Front Aging Neurosci | Authors: Wei Sun | Species: mouse | Application: WB,IF - Liquiritin, a novel inhibitor of TRPV1 and TRPA1, protects against LPS-induced acute lung injury.
Journal: Cell Calcium | Authors: Zhenhong Liu | Species: mouse,human | Application: WB - Transient receptor potential channels TRPA1 and TRPV1 are involved in chronic pain relief via Pulsed Radiofrequency in Rheumatoid Arthritis rat
Journal: Neuropharmacology | Authors: Ying-di Wang | Species: rat | Application: WB,IF - Transient receptor potential ankyrin 1 (TRPA1) positively regulates imiquimod-induced, psoriasiform dermal inflammation in mice.
Journal: J Cell Mol Med | Authors: Yan Zhou | Species: human | Application: WB - Study on the effect and mechanism of sesamin on P2X3-related cheek allergic contact dermatitis in mice induced by SADBE stimulation.
Journal: Neuropharmacology | Authors: Xiaoqian Ma | Species: mouse | Application: WB,IF - Validation of TRPA1 and TRPV1 Antibodies for Expression Detection in Mammalian Cells and Tissues.
Journal: J Neurochem | Authors: M. de las Casas | Species: mouse | Application: WB,IHC,IF - Menthol from Mentha piperita Suppresses the Milk Production of Lactating Mammary Epithelial Cells In Vivo and In Vitro.
Journal: Mol Nutr Food Res | Authors: Norihiro Suzuki | Species: mouse | Application: WB,IF - The Effectiveness of Wogonin on Treating Cough Mice With Mycoplasma Pneumoniae Infection.
Journal: Front Mol Biosci | Authors: Mingchuan Liang | Species: mouse | Application: WB - L-carnitine alleviates synovitis in knee osteoarthritis by regulating lipid accumulation and mitochondrial function through the AMPK-ACC-CPT1 signaling pathway
Journal: J Orthop Surg Res | Authors: Taiyang Liao | Species: rat | Application: WB - Inhibition of TRPA1 attenuates oxidative stress-induced damage after traumatic brain injury via the ERK/AKT signaling pathway.
Journal: Neuroscience | Authors: Xin-Jie Yang | Species: mouse | Application: WB - Effect of Pulsed Radiofrequency with Various Time-Course on Rheumatoid Arthritis Joint Pain in Rats.
Journal: J Pain Res | Authors: Xin Li | Species: rat | Application: WB,IF - Exploring the Analgesic Initiation Mechanism of Tuina in the Dorsal Root Ganglion of Minor CCI Rats via the TRPV1/TRPA1-cGMP Pathway
Journal: Pain Res Manag | Authors: Zhenjie Yang | Species: rat | Application: WB - Co-exposure to polystyrene microplastics and di-(2-ethylhexyl) phthalate aggravates allergic asthma through the TRPA1-p38 MAPK pathway
Journal: Toxicol Lett | Authors: Qi Han | Species: mouse | Application: IHC - Pharmacogenetic inhibition of lumbosacral sensory neurons alleviates visceral hypersensitivity in a mouse model of chronic pelvic pain.
Journal: PLoS One | Authors: Alison Xiaoqiao Xie | Species: mouse | Application: IF - Yanghe decoction attenuated pain hypersensitivity induced by michigan cancer foundation-7 injection in rats with bone metastases from breast cancer by inhibiting transient receptor potential ankyrin 1
Journal: J Tradit Chin Med | Authors: Gong Hui | Species: rat | Application: WB - Functional expression of TRPA1 channel, TRPV1 channel and TMEM100 in human odontoblasts.
Journal: J Mol Histol | Authors: Yangqiu Liu | Species: human | Application: WB,IHC,IF - UVR Promotes Keratinocyte Phagocytosis and Skin Pigmentation Through TRPA1 Channels.
Journal: Clin Cosmet Investig Dermatol | Authors: Ying Liu | Species: human | Application: WB - Chenodeoxycholic acid activates the TGR5/TRPA1-5-HT pathway to regulate intestinal motility in breastfed infants and mouse models
Journal: Transl Pediatr | Authors: Zihong Shan | Species: mouse | Application: WB
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