CaMKII Gamma Polyclonal antibody 12666-2-AP proteintech
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SKU
12666-2-AP
| Catalog Number | 12666-2-AP |
| Synonyms | CAMK2G, CaMK-II subunit gamma, CAMK-II, CAMK, CaM kinase II subunit gamma |
| Host | Rabbit |
| Reactivity | human, mouse, rat |
| Form | Liquid |
| Formulation | PBS, Azide, Glycerol |
| Applications | WB, IHC, IF/ICC, IP, CoIP, ELISA |
| Applications | WB, IHC, IF/ICC, IP, ELISA |
| Host / Isotype | Rabbit / IgG |
| Tested Applications — Positive WB detected in | SMMC-7721 cells, human brain tissue |
| Tested Applications — Positive IP detected in | mouse heart tissue |
| Tested Applications — Positive IHC detected in | mouse heart tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Tested Applications — Positive IF/ICC detected in | HepG2 cells |
| Recommended Dilutions — Western Blot (WB) | WB : 1:1000-1:4000 |
| Recommended Dilutions — Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Recommended Dilutions — Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Recommended Dilutions — Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Positive WB detected in | SMMC-7721 cells, human brain tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CatNo: Ag3348 Product name: Recombinant human CAMK2G protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 218-527 aa of BC034044 Sequence: DQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITADQALKHPWVCQRSTVASMMHRQETVECLRKFNARRKLKGAILTTMLVSRNFSVGRQSSAPASPAASAAGLAGQAAKSLLNKKSDGGVKKRKSSSSVHLMEPQTTVVHNATDGIKGSTESCNTTTEDEDLKVRKQEIIKITEQLIEAINNGDFEAYTKICDPGLTSFEPEALGNLVEGMDFHKFYFENLLSKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWLNVHYHCSGAPAAPLQ Predict reactive species |
| Full Name | calcium/calmodulin-dependent protein kinase II gamma |
| Calculated Molecular Weight | 527 aa, 59 kDa |
| Observed Molecular Weight | 59 kDa |
| GenBank Accession Number | BC034044 |
| Gene Symbol | CAMK2G |
| Gene ID (NCBI) | 818 |
| RRID | AB_2068213 |
| Conjugate | Unconjugated |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13555 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA. |
| IF protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| IHC protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| IP protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| WB protocol for CaMKII Gamma antibody 12666-2-AP | Download protocol |
| mouse | IF Development Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse. Authors - Chang Heng-Yu HY View Article |
| rat | WB Adv Healthc Mater Bioelectrically Reprogramming Hydrogels Rejuvenate Vascularized Bone Regeneration in Senescence Authors - Tianli Wu View Article |
| human | WB,IF Aging (Albany NY) CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis. Authors - Linlin Yang KD Validated View Article |
| Citations | 48 |
| Dilutions | WB : 1:1000-1:4000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:200-1:800 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Clone Number | - |
| Conjugation | Unconjugated |
CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology. Protocols Product Specific Protocols IF protocol for CaMKII Gamma antibody 12666-2-AP Download protocol IHC protocol for CaMKII Gamma antibody 12666-2-AP Download protocol IP protocol for CaMKII Gamma antibody 12666-2-AP Download protocol WB protocol for CaMKII Gamma antibody 12666-2-AP Download protocol Standard Protocols Click here to view our Standard Protocols
Publications
- JAKMIP1, a Novel Regulator of Neuronal Translation, Modulates Synaptic Function and Autistic-like Behaviors in Mouse.
Journal: Neuron | Authors: Jamee M Berg | Species: mouse | Application: WB - Bioelectrically Reprogramming Hydrogels Rejuvenate Vascularized Bone Regeneration in Senescence
Journal: Adv Healthc Mater | Authors: Tianli Wu | Species: rat | Application: WB - Cardiac-Specific Overexpression of CREM-IbΔC-X via CRISPR/Cas9 in Mice Presents a New Model of Atrial Cardiomyopathy with Spontaneous Atrial Fibrillation
Journal: Transl Res | Authors: Jiuzhou Chen | Species: mouse | Application: WB - PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.
Journal: Oncotarget | Authors: Chao Li | Species: human | Application: WB - Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse.
Journal: Development | Authors: Chang Heng-Yu HY | Species: mouse | Application: IF - CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis.
Journal: Aging (Albany NY) | Authors: Linlin Yang KD Validated | Species: human | Application: WB,IF - The delta subunit of the GABAA receptor is necessary for the GPT2-promoted breast cancer metastasis
Journal: Theranostics | Authors: Na Li | Species: human | Application: WB - Mas-Related G Protein-Coupled Receptor Member D Sustains Hypertension.
Journal: MedComm (2020) | Authors: Kun Zhao | Species: rat | Application: CoIP - TRPV1 alleviates osteoarthritis by inhibiting M1 macrophage polarization via Ca2+/CaMKII/Nrf2 signaling pathway.
Journal: Cell Death Dis | Authors: Zhongyang Lv | Species: rat | Application: WB - Wnt5a-induced M2 polarization of tumor-associated macrophages via IL-10 promotes colorectal cancer progression.
Journal: Cell Commun Signal | Authors: Qing Liu | Species: human | Application: WB - A flaxseed oil body-based delivery system integrating calcium overload and lipid peroxidation for immunogenic cell death-driven immunotherapy.
Journal: Mater Today Bio | Authors: Xiaoxiao Chen | Species: mouse | Application: WB - Bioelectrically Reprogramming Hydrogels Rejuvenate Vascularized Bone Regeneration in Senescence
Journal: Adv Healthc Mater | Authors: Tianli Wu | Species: rat | Application: WB - CaMKII nucleates an osmotic protein supercomplex to induce cellular bleb expansion.
Journal: EMBO J | Authors: Yuki Fujii | Species: human | Application: WB,IF - CaMKII protein expression and phosphorylation in human skeletal muscle by immunoblotting: isoform specificity
Journal: Free Radic Biol Med | Authors: Miriam Martinez-Canton | Species: human | Application: WB - In vitro and in vivo inhibition of the host TRPC4 channel attenuates Zika virus infection
Journal: EMBO Mol Med | Authors: Xingjuan Chen | Species: mouse | Application: WB - Nonthermal radiofrequency radiation promotes hematopoietic stem and progenitor cells function by regulating Ca(2+) efflux.
Journal: Stem Cell Res Ther | Authors: Zhichun Lv | Species: mouse | Application: WB - Near-Infrared II Fluorescence Imaging and Image-Guided siRNA Therapy of Atherosclerosis
Journal: J Med Chem | Authors: Jialu Gao | Species: mouse | Application: WB - HOCPCA Exerts Neuroprotection on Retinal Ganglion Cells by Binding to CaMKIIα and Modulating Oxidative Stress and Neuroinflammation in Experimental Glaucoma
Journal: Neurosci Bull | Authors: Panpan Li | Species: mouse | Application: WB,IF - HOCPCA Exerts Neuroprotection on Retinal Ganglion Cells by Binding to CaMKIIα and Modulating Oxidative Stress and Neuroinflammation in Experimental Glaucoma
Journal: Neurosci Bull | Authors: Panpan Li | Species: mouse | Application: WB,IF - The phosphorylation of Smad3 by CaMKIIγ leads to the hepatocyte pyroptosis under perfluorooctane sulfonate exposure
Journal: Ecotoxicol Environ Saf | Authors: Siyu Ren | Application: WB,IP - Orally administered sodium nitrite prevents the increased α-1 adrenergic vasoconstriction induced by hypertension and promotes the S-nitrosylation of calcium/calmodulin-dependent protein kinase II
Journal: Biochem Pharmacol | Authors: Gustavo H Oliveira-Paula | Species: mouse,rat | Application: WB - PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of Stathmin1.
Journal: Oncotarget | Authors: Chao Li | Species: human | Application: WB - Cardiac-Specific Overexpression of CREM-IbΔC-X via CRISPR/Cas9 in Mice Presents a New Model of Atrial Cardiomyopathy with Spontaneous Atrial Fibrillation
Journal: Transl Res | Authors: Jiuzhou Chen | Species: mouse | Application: WB - Distinct Metabolomic and Proteomic Signatures of Early Brain Damage Triggered by the Entrance Plateau Versus the Spread-Out Bragg Peak of Proton Radiation.
Journal: Cells | Authors: Keman Liao - CAMKIIγ is a targetable driver of multiple myeloma through CaMKIIγ/ Stat3 axis.
Journal: Aging (Albany NY) | Authors: Linlin Yang | Species: human | Application: WB,IF - The novel peptide athycaltide-1 attenuates Ang II-induced pathological myocardial hypertrophy by reducing ROS and inhibiting the activation of CaMKII and ERK1/2
Journal: Eur J Pharmacol | Authors: Xi Zheng | Species: human | Application: WB - Evaluation of the synergistic effects of epigallocatechin-3-gallate-loaded PEGylated-PLGA nanoparticles with nimodipine against neuronal injury after subarachnoid hemorrhage
Journal: Front Nutr | Authors: Xianguang Yang | Species: mouse | Application: IHC - A new specific GluN2B partial antagonist ameliorates brain injury caused by ischemic stroke in rats
Journal: Bioorg Chem | Authors: Yanhua Fan | Species: human,rat | Application: WB - PDCD4 exacerbates atrial fibrillation by inducing mitochondrial fission and activating Ca(2+)/CaMKII signaling via ROS.
Journal: Cell Signal | Authors: Qin Liu - Pin1 Is Regulated by CaMKII Activation in Glutamate-Induced Retinal Neuronal Regulated Necrosis.
Journal: Front Cell Neurosci | Authors: Shuchao Wang | Species: human | Application: WB - Mechanical stretch-mediated fibroblast activation: The pivotal role of Piezo1 channels
Journal: Biochim Biophys Acta Mol Cell Res | Authors: Shiwei Tu | Species: mouse | Application: IF - iTRAQ-based proteomic analysis of dioscin on human HCT-116 colon cancer cells.
Journal: Proteomics | Authors: Hao Chen | Species: human | Application: WB - Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.
Journal: J Biol Chem | Authors: Qingnan Yan | Species: mouse | Application: WB - Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse.
Journal: Development | Authors: Chang Heng-Yu HY | Species: mouse | Application: IF - Kilohertz High-Frequency Electrical Stimulation Effectively Mitigates Hyperalgesia in Mice With Neuropathic Pain Through Regulation of the Calcium/Calmodulin-Dependent Protein Kinase II/N-Methyl-D-Aspartate 2B Signaling Pathway
Journal: Neuromodulation | Authors: Kexin Fang | Species: mouse | Application: WB,IF - Guaiane-type sesquiterpenoids from patchouli oil and their potential anti-depressive effects.
Journal: Phytochemistry | Authors: Qi Zheng | Species: rat | Application: WB - Identification of TRAF2, CAMK2G, and TIMM17A as biomarkers distinguishing mechanical asphyxia from sudden cardiac death base on 4D-DIA Proteomics: A pilot study
Journal: J Pharm Biomed Anal | Authors: Yuebing Huang | Species: rat | Application: WB,IHC - Silver Needle Thermotherapy Regulates TRPV1 to Alleviate Endoplasmic Reticulum Stress and Relieve Myofascial Pain.
Journal: J Pain Res | Authors: Xinyao Chen | Species: rat | Application: WB,IF - Immunohistochemical distribution of Ca2+/calmodulin-dependent protein kinase II subunits in the rat carotid body
Journal: Acta Histochem | Authors: Hiroki Saito | Species: rat | Application: IF - Silver Needle Thermal Therapy Improves Mitochondrial Injury in the Skeletal Muscle of MPS Rats by Inhibiting the TRPV1/CaMKII Pathway
Journal: Physiol Res | Authors: Y Huang | Application: WB - TAK-242 alleviates diabetic cardiomyopathy via inhibiting pyroptosis and TLR4/CaMKII/NLRP3 pathway
Journal: Open Life Sci | Authors: Xiaolong Zhao | Species: rat | Application: WB - CAMK2G Promotes Neuronal Differentiation and Inhibits Migration in Neuroblastoma
Journal: J Pediatr Surg | Authors: Yujie Ma | Species: human | Application: WB - RIP3 facilitates necroptosis through CaMKII and AIF after intracerebral hemorrhage in mice.
Journal: Neurosci Lett | Authors: Yang Xu | Species: mouse | Application: WB - Vericiguat Alleviates Atrial Electrical and Structural Remodeling in Rats with Atrial Fibrillation.
Journal: Anatol J Cardiol | Authors: Xia Zhang - The Effects of Different Developmental Stages on Bone Regeneration of Periodontal Ligament Stem Cells and Periodontal Ligament Cell Sheets In Vitro and Vivo
Journal: Stem Cells Dev | Authors: Xin Shao | Species: human | Application: WB - Wnt5a protects motor neurons in amyotrophic lateral sclerosis by regulating the Wnt/Ca2+ signaling pathway
Journal: Am J Transl Res | Authors: Jinmeng Liu | Species: mouse | Application: WB - Mechanism of action of l-theanine on cerebellar granule cell migration via signaling pathways.
Journal: Biosci Biotechnol Biochem | Authors: Mai Ibrahim | Species: mouse | Application: WB - Azoramide ameliorated tachypacing-induced injury of atrial myocytes differentiated from human induced pluripotent stem cell by regulating endoplasmic reticulum stress
Journal: Stem Cell Res | Authors: Weilun Miao | Species: human | Application: WB - STING‑regulated Ca²⁺-TGF‑β axis drives airway remodeling and lung function decline in asthma: a multi‑omics mechanistic study.
Journal: Biol Direct | Authors: Zhiqin Zhu - CACNA2D1 promotes ovulation by regulating ovarian immune cell infiltration.
Journal: BMC Vet Res | Authors: Conghui Guo
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