Mouse CD25/IL-2RA ELISA Kit KE10208 proteintech
$299.00
In stock
SKU
KE10208
| Product name | Mouse CD25/IL-2RA ELISA Kit |
| Synonyms | CD25, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha, Il2ra |
| Reactivity | Mouse |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 7.8 pg/mL |
| Range | 46.88-3000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 16184 |
| Sample Type | Average Range |
| Mouse serum | 77% 73%-83% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 1,461.8 55.5 3.8 |
| 2 | 16 381.4 17.2 4.5 |
| 3 | 16 175.6 7.7 4.4 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
CD25, also known as the α chain of the IL-2 receptor (IL-2Rα), is a key regulator of T cell function. It is expressed in activated T cells and has been implicated in regulating various immune and autoimmune responses. Its deficiency has been associated with autoimmune phenomena including autoimmune enteropathy and bullous pemphigoid. The soluble CD25 can be found in the serum and may have implications for human disease in both cancer and chronic infection. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD25, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha, Il2ra FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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